Type

Recombinant

Description

Lin28isaRNA-bindingproteinthatbelongstoadiversefamilyofstructurally-relatedtranscriptionfactors.Lin28isfoundabundantlyinembryonicstemcells(ESCs),andtoalesserextentinplacentaandtestis.Lin28hasbeenshowntoblocklet-7 microRNAprocessingandmaturation,anecessarystepinthedifferentiationofstemcellsandcertaincancercelllines.TogetherwithSox2,Oct4,andNanog,Lin28caninducethereprogrammingofprimaryhumanfibroblaststoapluripotentstate.Lin28andotherregulatoryproteinscanbeintroducedintocellsbyDNAtransfection,viralinfection,ormicroinjection.ProteintransductionusingTATfusionproteinsrepresentsanalternativemethodologyforintroducingproteinsintoprimary,aswellastransformed,cells.RecombinantHumanLin28-TATisa24.4 kDaproteincontaining222 aminoacidresidues,includinga13-residueC-terminalTATpeptide.

AminoAcidSequence

GPSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGP

Source

E.coli

Purity

90%

BIOLOGicalActivity

MeasuredbyitsABIlitytoinducefluorescenceinLin28reportercells(293 cellstransfectedwithfluorescentproteingenesunderLin28control).Optimumactivitywasachievedat20 µg/mlafterincubationfor72 hr.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C