反应生物学/cIAP1-BIR3(GST)/APT-11-371

价格
¥6400.00
货号:APT-11-371
浏览量:127
品牌:Reaction Biology
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商品描述

DESCRIPTION:

Human recombinant cIAP-BIR3.

ACCESSION #:NM_001166.4

Uniprot Link

INCLUDES AMINO ACIDS:258-363

TAG(S):N-terminalGST tagC-terminal StrepII tag

MW:40.3kDa

EXPRESSION SYSTEM:E.coli

PURITY: >85% by SDS-PAGE

ASSAY CONDITIONS:

RBC’s cIAP1-BIR3 domain displays bindingaffinity for Smac/DIABLO peptide. Reactions were performed with 20nMFAM-labelled peptide at room temperature in 50mM HEPES, pH 7.5, 200mM NaCl,0.01% Triton X100. The resulting polarization values (Ex. 480 nm/Em. 535 nm)was read following a 30 minute incubation.(See figure, below.)

SUPPLIED AS: _ µg/µL in 50 mM Tris HCl, pH 7.5,500 mM NaCl, 1 mM TCEP, 10% glycerol

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen,for example in a dry ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but verylow volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.

AMOUNT: 100ug

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Substantial Discounts Available on Bulk Quantities
Reaction Biology Corp.是一家专业从事早期药物发现研究的行业***和创新者。成立于2001年,Reaction Biology Corp.提供了广泛的服务,服务包括对激酶和后生生化和基于细胞的高通量筛选(HTS)和选择性复合分析的可靠的分析技术。Reaction Biology Corp.还提供相关的HTS实验试剂,包括高纯度和特化的表观遗传蛋白。每年有数百万的HTS和分析反应在我们的协助下完成。全球有超过750家公司,包括制药、生物技术、学术和非盈利性实验室,在使用Reaction Biology Corp.的生化和细胞分析服务并购买表观遗传蛋白。