RibonucleaseA,PurifiedAlyophilizedpreparationequivalenttoCode:RASE.Containssomeaggregatesasaresultoflyophilizationbutshowsnodifferenceinspecificactivity.Storeat2-8°C.PROTECTFROMMOISTURE.≥3,000unitspermgdryweightSource:BovinePancreas

I.U.B.:3.1.27.5

Pancreaticribonuclease(RNaseI)catalyzescleavageofthephosphodiesterbondbetweenthe5"-riboseofanucleotideandthephosphategroupattachedtothe3"-riboseofanadjacentpyrimidinenucleotideforminga2",3"-cyclicphosphatewhichmaythenbehydrolyzedtothecorresponding3"-nucleosidephosphate.RibonucleaseAhasamolecularweightof13,700daltons.ItoperatesinanoptimumpHrangeof7.0-7.5.

RibonucleaseBhasamolecularweightof14,700±300daltons.ItisaglycoproteinwhichpossessesanaminoacidcompositionindistinguishablefromthatofRNaseA.Itcontains6residuesofmannoseand2residuesofN-acetylglucosaminepermolecule.ItisaglycosylatedderivativeofRNaseA.

RibonucleaseisinhibitedbyheavymetalionsanditiscompetitivelyinhibitedbyDNA.SinceMolecularBIOLOGyGradeRNaseA(ProductCode:RPDF)isessentiallyfreeofDNaseandproteaseactivities,thisproductisusefulinremovingRNAfromDNAinnucleicacidworkandwhereotherenzymesareusedorwhereintactproteinsmustberecovered.

StABIlity/Storage:MolecularBiologyGradeproduct(ProductCode:RPDF)isstableatleast2yearsat2-8°Cor-20°C.OthergradesofRNaseAandRNaseBarestable2-3yearsat2-8°C.R,RB,andRAF:Storeat2-8°Candprotectfrommoisture.ProductCode:RASE:Storeat-20°Ctomaintainmonomericform.ProductCode:RPDF:Storeat2-8°Corat-20°C.

UnitDefinition:OneKalnitskyUnitcausesanincreaseinabsorbanceof1.0at260nmat37°CandpH5.0whenyeastribosomalRNAishydrolyzedtoacidsolubleoliognucleotides.OneKunitzunitequals50Worthingtonunits.

TechnicalNotes:Specialcareshouldbegiventohandlingoftheenzymebecauseofitsaffinityforglasssurfaces.Theenzymeremainsactivebutaggregatesuponlyophilizationandinsolutionattemperatures≥2°Catlowionicstrength.HeatingsolutionsofRNaseAtoinactivateDNasemaynotbesatisfactorysinceRNaseactivitymaybelostifprecipitateformationoccursandheattreatedDNasemayreactivateovertime.ProductCode:RPDFissuitableassuppliedforapplicationsrequiringminimalDNaseandproteaselevelsandneedsnofurthertreatment.ProductCode:RAFcanbeusedwithouttreatmentinsomeapplications.Toheat-treatRAF,use10mMacetatepH5.0withorwithout15mMCaCl₂for15minutesat100°Corlongerat80°C.ProductmayprecipitateifheatedatneutralpH.HeattreatmentofProductCode:RASEwillprecipitateproductduetothepresenceofphosphate.

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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。