ProteinaseKAlyophilizedpowder.PurifiedtoremoveDNaseandRNase.Storeat2-8°C.≥20unitspermgdryweightSource:Tritirachiumalbumlimber

I.U.B.:3.4.21.64

ProteinaseK(PROK)isaserineproteasewithbroadspecificitytowardsaliphatic,aromaticandotherhydrophobicaminoacids.PROKhasamolecularweightof27,000daltonsandisCa²⁺dependent.ItisnotinactivatedbymetalionchelatingagentssuchasEDTA,sulfhydrylreagents,PCMB,TLCK,orTPCK.Italsoretainsactivityin0.5%SDS.ItcanbeinhibitedbyPMSForDFP.

StABIlity/Storage:Thelyophilizedpowderisstableforoneyearat2-8°C.Solutionsin50mMTris-HCl,pH8.0with1mMCaCl₂arestableformonthsat2-8°C.Storeat2-8°C.

UnitDefinition:OneUnitreleasesonemicromoleofFolinpositiveaminoacids,measuredastyrosine,at37°C,pH7.5,usingureadenaturedhemoglobinasthesubstrate.

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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。