DeoxyribonucleaseIPartiallypurified.Alyophilizedpowder.Storeat2-8°C.≥2,000KunitzunitspermgdryweightSource:BovinePancreas

I.U.B.:3.1.21.1

Bovinepancreaticdeoxyribonucleaseisanendonucleasewhichsplitsphosphodiesterlinkages,preferentiallyadjacenttoapyrimidinenucleotideyieldingpolynucleotideswithfreehydroxylgroupatthe3"positionandphosphategroupatthe5"position.Theaveragechainlengthofalimitdigestisatetranucleotide.

Uses:WorthingtonoffersDNaseatdifferentlevelsofpurityfordifferentapplications.ProductCodes:DPRFandDPRFSarebothespeciallydesignedforMolecularBIOLOGyapplicationsandcontainthelowestlevelsofRNaseandproteaseactivity.TheyarebothsuitableforuseintechniquesrequiringdigestionofDNAintherecoveryofintactRNAorwheretheintegrityofstructuralproteinsorenzymesmustbemaintained.Applicationshaveincludednicktranslation,DNAmapping,isolationofnuclearRNAandprotein,plasmidconstruction,andRNApolymerasesynthesisofRNAprobesandRT-PCR.DNaseisalsousedintissuecultureworktodigestDNAfromdamagedcellstherebyreducingviscosity,andremovingmembraneboundDNAfragments.WorthingtonCodes:DPandDCLSaresuitablefortheseapplications.

StABIlity/Storage:Whenproperlystored,allgradesofWorthingtondeoxyribonucleasearestablefor2-3years.RecommendedstoragetemperatureforallgradesofWorthingtonDNasesis2-8°C.ProductcodeDPRFSmaybestoredat-20°C.Forlongtermstorageinsolution,ProductCodesDandDPFFmaybedissolvedin5mMacetate,1mMcalcium,pH4.5andstoredinsingleusealiquotsat-20°Cor-70°Cforuptooneyear.Onlyfreezeandthawonce;thawedaliquotsarestablerefrigeratedatleastseveralweeks.Additionof50%glycerolwillmaintainaliquidstateat-20°Cwithoutaffectingstabilityandmaterialin50%glycerolcanberemovedandreturnedto-20°Crepeatedly.DPRFisunusuallystableduetotheabsenceofprotease.ForlongtermstorageofDPRFafterreconstitution,usewateroranybufferpH4.0to9.0exceptphosphate;avoidcalciumchelators;add50%glycerolforstorageasliquidat-20°C;aliquotinsingleusecontainers;onlyfreezeandthawonce;thawedaliquotsarestablerefrigeratedatleastseveralweeks.

UnitDefinition:1Unitcausesanincreaseinabsorbanceat260nmof0.001perminutepermlat25°CwhenactinguponhighlypolymerizedDNAatpH5.0.Note:Kunitzunitsasreportedbyothersupplierscanbe2to4timeshigherthanKunitzunitsasmeasuredatWorthington.AsmeasuredatWorthington,oneKunitzunitdigests1µgoflamBDaDNAin10minutesat37°Cin50mMTris,1mMMg²⁺,1mMCa²⁺,pH7.8ina50µlreaction.CorrelationofdigestionunitswithKunitzunitsisdifferentforotherDNAandbuffersystems.

TechnicalNote:ProductCodeDPRF:Eachvialcontainsapproximately2mgglycineand2µmolescalciumper10,000unitsofDNaseI.Dissolvingtheentirevialin5mlprovidestheequivalentofa1mg/mlsolution.

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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。