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蚂蚁淘在线 / 品牌中心 / StressMarq / StressMarq/StressXpress® SOD Activity Kit/SKT-214-192/2-x-96-well

StressMarq/StressXpress® SOD Activity Kit/SKT-214-192/2-x-96-well

价格
￥8480.00
货号：SKT-214-192
浏览量：127
品牌：StressMarq
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商品描述

Overview:

ProductNameSODActivityKit
Description

Quantitativecolorimetricmeasurementofsuperoxidedismutaseactivity

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesCelllysates,EDTAPlasma,Erythrocytes,HeparinPlasma,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeDirectEnzymeActivityAssay
UtilityColorimetricassayusedtoquantitativelymeasureSODactivityinavarietyofsamples.
Sensitivity0.044U/ml
AssayRange0.0625-4U/ml
PrecisionIntraAssayPrecision:ThreesamplesdilutedinAssayBufferwereruninreplicatesof20inanassay.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-0.407U/mL,4.6%CVSample2-0.726U/mL,7.3%CVSample3-1.203U/mL,16.8%CVInterAssayPrecision:ThreesamplesdilutedinAssayBufferwereruninduplicatesinsixteenassaysrunovermultipledaysbyfouroperators.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-0.356U/mL,10.5%CVSample2-0.653U/mL,6.1%CVSample3-1.277U/mL,13.8%CV
NumberofSamples89samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeActivityKits
AssayOverviewTheSuperoxideDismutase(SOD)ActivityKitisdesignedtoquantitativelymeasureSODactivityinavarietyofsamples.TheassaymeasuresalltypesofSODactivity,includingCu/Zn,Mn,andFeSODtypes.AbovineerythrocyteSODstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffofthestandardcurve.SamplesaredilutedinourspeciallycoloredSampleDiluentandaddedtothewells.TheSubstrateisaddedfollowedbyXanthineOxidaseReagentandincubatedatroomtemperaturefor20minutes.TheXanthineOxidasegeneratessuperoxideinthepresenceofoxygen,whichconvertsacolorlesssubstrateintheDetectionReagentintoayellowcoloredproduct.Thecoloredproductisreadat450nm.IncreasinglevelsofSODinthesamplescausesadecreaseinsuperoxideconcentrationandareductioninyellowproduct.TheactivityoftheSODinthesampleiscalculatedaftermakingasuitablecorrectionforanydilution,usingsoftwareavailablewithmostplatereaders.TheresultsareexpressedintermsofunitsofSODactivitypermL.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-214AClear96wellHalfAreaPlates2Plates
SKC-214BSuperoxideDismutaseStandard1Vial
SKC-214CAssayBuffer50ml
SKC-214DXanthineOxidaseBuffer6ml
SKC-214EXanthineOxidaseConcentrate225µl
SKC-214FSubstrateDiluent12ml
SKC-214GSubstrateConcentrate1.1ml
CiteThisProductSODActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-214)

BIOLOGicalDescription

AlternativeNamesCu/ZnSODActivityKit,SOD1ActivityKit,MnSODActivityKit,SOD2ActivityKit,EC-SODActivityKit,SOD3ActivityKit
ResearchAreasCancer,Alzheimer"sDisease,Atherosclerosis,CardiovascularSystem,Neurodegeneration,Neuroscience,OxidativeStress,Parkinson"sDisease
ScientificBackgroundShort-livedandhighlyreactiveoxygenspecies(ROS)suchasO2·-(superoxide),·OH(hydroxylrADIcal),andH2O2(hydrogenperoxide)arecontinuouslygeneratedinvivo.Intherestingstate,thebalancebetweenantioxidantsandoxidantsissufficienttopreventthedisruptionofnormalphysiologicfunctions;however,eitherincreasesinoxidantsordecreasesinantioxidantscandisruptthisbalancegivingrisetoelevatedlevelsofreactiveoxygenspecies(ROS)(1,2).ThecellularlevelsofROSarecontrolledbyantioxidantenzymesandsmallmoleculeantioxidants.Themajorantioxidantenzymes,superoxidedismutases(SODs),includingcopper-zincsuperoxidedismutase(Cu/ZnSOD,SOD1),manganesesuperoxidedismutase(MnSOD,SOD2)andextracellularsuperoxidedismutase(EC-SOD,SOD3),allplaycriticalrolesinscavengingO2·-.DecreasedSODactivityresultsinelevatedlevelofsuperoxidewhichinturnleadstodecreasedNObutincreasedperoxynitriteconcentrations.ThemajorintracellularSODisa32-kDcopperandzinccontaininghomodimer(Cu/ZnSOD).ThemitochondrialSOD(MnSOD)isamanganese-containing93-kDhomotetramerthatissynthesizedinthecytoplasmandtranslocatedtotheinnermatrixofmitochondria.EC-SODistheprimaryextracellularSODenzymeandishighlyexpressedinmanyorgans.IncreasedSODactivitylevelsareseeninDownsSyndrome(3)whiledecreasedactivityisseenindiabetes,Alzheimer’sdisease,rheumatoidarthritis,Parkinson’sdisease,uremicanemia,atherosclerosis,somecancers,andthyroiddysfunction(3-8).
References1.Liocher,SIandFridovich,I.FreeRad.Biol.Med.,2007,42:1465-1469.
2.Imlay,JA.AnnRev.Bichem.,2008,77:755-776.
3.Torsdottir,G.etal.J.Neurol.Sci.2010.299(1-2):51-54.
4.Giacco,F&Brownlee,M.Circ.Res.2010.107(9):1058-1070.
5.Bae,S-C,etal.J.Amer.Coll.Nutr.2003.22(4):311-315.
6.Akbostanci,MC,etal.ActaNeurol.Belg.2001.101:180-183.
7.Shainkin-Kestenbaum,R,etal.Nephron.1990.55(3):251-253.
8.Saito,T.HokkaidoIgakuZasshi.1987.62(2):257-268.

ProductImages

<p>Typical Standard Curve for SOD Activity Kit (Enzyme Activity Assay) StressXpress® – SKT-214. Assay Type: Direct Enzyme. Detection Method: Colorimetric Assay. Assay Range: 0.0625 – 4 U/ml.</p>

TypicalStandardCurveforSODActivityKit(EnzymeActivityAssay)StressXpress®–SKT-214.AssayType:DirectEnzyme.DetectionMethod:ColorimetricAssay.AssayRange:0.0625–4U/ml.

<p>Linearity was determined by taking two samples, one with a high known SOD activity and the other with a lower SOD activity and mixing them in given ratios. The measured activities were compared to the expected values based on the ratios used.</p>

Linearitywasdeterminedbytakingtwosamples,onewithahighknownSODactivityandtheotherwithalowerSODactivityandmixingthemingivenratios.Themeasuredactivitieswerecomparedtotheexpectedvaluesbasedontheratiosused.

<p>Short-lived and highly reactive oxygen species (ROS) such as O2·-(superoxide), ·OH (hydroxyl radical), and H2O2 (hydrogen peroxide) are continuously generated in vivo. The cellular levels of ROS are controlled by antioxidant enzymes and small molecule antioxidants. The major antioxidant enzymes, superoxide dismutases (SODs), including copper-zinc superoxide dismutase (Cu/ZnSOD, SOD1), manganese superoxide dismutase (MnSOD, SOD2) and extracellular superoxide dismutase (EC-SOD, SOD3), all play critical roles in scavenging O2·-.</p>

Short-livedandhighlyreactiveoxygenspecies(ROS)suchasO2·-(superoxide),·OH(hydroxylradical),andH2O2(hydrogenperoxide)arecontinuouslygeneratedinvivo.ThecellularlevelsofROSarecontrolledbyantioxidantenzymesandsmallmoleculeantioxidants.Themajorantioxidantenzymes,superoxidedismutases(SODs),includingcopper-zincsuperoxidedismutase(Cu/ZnSOD,SOD1),manganesesuperoxidedismutase(MnSOD,SOD2)andextracellularsuperoxidedismutase(EC-SOD,SOD3),allplaycriticalrolesinscavengingO2·-.

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