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蚂蚁淘在线 / 品牌中心 / StressMarq / 应力MARQ/NpFR1/SIH-181-1MG/1-mg

应力MARQ/NpFR1/SIH-181-1MG/1-mg

价格
￥8500.00
货号：SIH-181-1MG
浏览量：127
品牌：StressMarq
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商品描述

Overview:

ProductNameNpFR1
Description

ReversIBLefluorescenceintensity-basedredoxsensor

MolecularFormulaC23H21N5O4
MolecularWeight431.45

Properties

StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
ProductTypeRedoxProbe
SolubilitySolubleinDMSO
SourceSynthetic
AppearanceRedSolid
SMILESC1(N(C(C2=CC5=C(C3=CC=CC1=C23)N(C4=NC(NC(C4=N5)=O)=O)CCC)=O)CCCC)=O
InChIInChI=1S/C23H21N5O4/c1-3-5-10-28-21(30)13-8-6-7-12-16(13)14(22(28)31)11-15-18(12)27(9-4-2)19-17(24-15)20(29)26-23(3
InChIKeyC5(C2=C1C(=CC4=C(C1=CC=C2)N(C3=NC(NC(C3=N4)=O)=O)CCC)C(N5CCCC)=O)=O
SafetyPhrasesClassification:Caution:Substancenotyetfullytested.SafetyPhrases:S22-DonotbreathedustS24/25-AvoidcontactwithskinandeyesS36/37/39-Wearsuitableprotectiveclothing,glovesandeye/faceprotection
CiteThisProductNpFR1(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SIH-181)

BIOLOGicalDescription

ResearchAreasCancer,OxidativeStress
ScientificBackgroundNpFR1,ornapthalimide-flavinredoxsensor1,isanovelflavinmoleculethatexhibitsagreaterthan100-foldincreaseinfluorescenceuponoxidation,andiseasilyreversible.Intheoxidativeform,isshowsamaximumabsorptionat395and463nm,maxadmissionat545nm.Treatmentwithamildreducingagent(includingsodiumthiosulfate,sodiumcyanoborohydride,DTTandglutathione)gavethereducedformwhichexhibited110-foldlowerabsorbance,and125-foldloweremission.Re-oxidationtoitsoriginalfluorescencecanbeachievedbyairorbyhydrogenperoxide.
References1.YeowJ.,KaurA.K.,AnscombM.D.,andNewE.J.(2014)Chem.Commun.50:8181-8184.

ProductImages

<p>Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 µM. Excitation: 405 or 488 nm (not shown). Emission: 490 – 600 nm, with peak at 545 nm.</p>

FluorescencebehaviorofNpFR1(SIH-181)intheoxidized(black)andreduced(red)forms,using50µM.Excitation:405or488nm(notshown).Emission:490–600nm,withpeakat545nm.

<p>Chemical structure of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

ChemicalstructureofNpFR1(SIH-181),areversiblefluorescenceintensity-basedredoxsensor.ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Fluorescence emission of NpFR1 (SIH-181, 5 mM, lex = 405 nm) with the incremental addition of sodium dithionite. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

FluorescenceemissionofNpFR1(SIH-181,5mM,lex=405nm)withtheincrementaladditionofsodiumdithionite.AllspectrawereacquiredinHEPESbuffer(100mM,pH7.4).ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Fluorescence response of NpFR1 (SIH-181) to cycles of oxidation and reduction. Reduction was achieved with sodium dithionite (100mM) followed by re-oxidation with 250mM H2O2. Spectra were recorded 5 min after the addition of reducing and oxidising agents. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

FluorescenceresponseofNpFR1(SIH-181)tocyclesofoxidationandreduction.Reductionwasachievedwithsodiumdithionite(100mM)followedbyre-oxidationwith250mMH2O2.Spectrawererecorded5minaftertheadditionofreducingandoxidisingagents.AllspectrawereacquiredinHEPESbuffer(100mM,pH7.4).ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Imaging of NpFR1 (SIH-181) in 3T3-L1 preadipocytes treated with (a) vehicle control (b) H2O2 (100 mM, 2 min), (c) NpFR1 (50 mM, 2 h) and (d) NpFR1 (50 mM, 2 h) followed by H2O2 (100 mM, 2 min). Scale bar represents 50 mm, lex = 405 nm. (e) Integrated emission from 510 nm to 610 nm. Values are the mean ratio generated from the intensity from five fields of cells. Error bars represent standard error measurement (s.e.m.). The layout of this image was modified for optimal display from the original Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

ImagingofNpFR1(SIH-181)in3T3-L1preADIpocytestreatedwith(a)vehiclecontrol(b)H2O2(100mM,2min),(c)NpFR1(50mM,2h)and(d)NpFR1(50mM,2h)followedbyH2O2(100mM,2min).Scalebarrepresents50mm,lex=405nm.(e)Integratedemissionfrom510nmto610nm.Valuesarethemeanratiogeneratedfromtheintensityfromfivefieldsofcells.Errorbarsrepresentstandarderrormeasurement(s.e.m.).ThelayoutofthisimagewasmodifiedforoptimaldisplayfromtheoriginalChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

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StressMarq硫黄素T测定硫黄素T是一种荧光染料，可与富含β片层的结构（例如α突触核蛋白原纤维中的结构）结合。结合后，染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液（新鲜制备，并通过0.2μm注射器过滤器过滤）。在PBS pH 7.4中稀释硫黄素T，使每孔中硫黄素T的最终浓度为25μM（每孔体积= 100μL）。板：Lumox 96多孔板（Sarstedt目录号94.6000.024）。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体（或两者）添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值，需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱（600 rpm）中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置：温度：37°C读取类型：阱扫描波长：450 nm处的激发和485 nmPMT 处的发射增益：每次读取自动闪烁：6次摇动：读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。