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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Overview:
| ProductName | HO-1ELISAKit |
| Description | Colorimetricdetectionofhemeoxygenase1 |
| SpeciesReactivity | Human |
| Platform | Microplate |
| SampleTypes | Celllysates,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitateHO-1concentrationinsamples. |
| Sensitivity | 0.21ng/ml |
| AssayRange | 0.781-50ng/mL |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | |||||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | |||||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add50µLofPre-TreatmentBuffertoallsampleandstandardwells.3.Add50µLofStandardandsampletoappropriatewells.4.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for2hours.5.Washplatefourtimeswith1XWashBuffer.6.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.7.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.8.Washplatefourtimeswith1XWashBufferasdescribedinstep5.9.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.10.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.11.Washplatefourtimeswith1XWashBufferasdescribedinstep5.12.Add100µLofTMBSubstratetoeachwell.13.Developtheplateinthedarkatroomtemperaturefor30minutes.14.Stopreactionbyadding100µLofStopSolutiontoeachwell.15.Measureabsorbanceonaplatereaderat450nm. | |||||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | HO-1ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-111) |
BIOLOGicalDescription
| AlternativeNames | Hemeoxygenase1ELISAKit,HemoxELISAKit,HMOX1ELISAKit,HO1ELISAKit,HO1ELISAKit,HSP32ELISAKit |
| ResearchAreas | Cancer,OxidativeStress |
| ScientificBackground | Heme-oxygenaseisaubiquitousenzymethatcatalyzestheinitialandrate-limitingstepsinhemecatabolismyieldingequimolaramountsofbiliverdin,ironandcarbonmonoxide.Biliverdinissubsequentlyconvertedtobilirubinandthefreeironissequesteredtoferritin(1).Theseproductshaveimportantphysiologicaleffectsascarbonmonoxideisapotentvasodilator;biliverdinandbilirubinarepotentantioxidants;andthefreeironincreasesoxidativestressandregulatestheexpressionofmanymRNAs(2).Therearethreeisoformsofheme-oxygenase,HO-1,HO-2andHO-3;howeverHO-1andHO-2arethemajorisoformsastheybothhavebeenidentifiedinmammals(3).HO-1,alsoknownasheatshockprotein32,isaninducIBLeisoformactivatedbymostoxidativestressinducers,cytokines,inflammatoryagentsandheatshock.HO-2isaconstitutiveisoformwhichisexpressedunderhomeostaticconditions.HO-1isalsoconsideredtobeacytoprotectivefactorinthatfreehemeishighlyreactiveandcytotoxic,andsecondly,carbonmonoxideisamediatorinhibitingtheinflammatoryprocessandbilirubinisascavengerforreactiveoxygen,bothofwhicharetheendproductsofhemecatalyzation(4).IthasalsobeenshownthatHO-1deficiencymaycausereducedstressdefense,apro-inflammatorytendency(5),susceptibilitytoatheroscleroticlesionformation(6),endothelialcellinjury,andgrowthretardation(7).Up-regulationofHO-1isthereforesaidtobeoneofthemajordefensemechanismsofoxidativestress(4). |
| References | 1.FrohM.etal.(2007)WorldJ.Gastroentereol13(25):3478-86. 2.ElbirtK.K.andBonkovskyH.L.(1999)ProcAssocAmPhysicians111(5):348-47. 3.MainesM.D.,TrakshelG.M.,andKuttyR.K.(1986)JBiolChem261:411–419. 4.BrydunA.,etal.(2007)HypertensRes30(4):341-8. 5.PossK.D.andTonegawaS.(1997).ProcNatlAcadSciUSA.94:10925–10930. 6.YetS.F.,etal.(2003)FASEBJ.17:1759–1761. 7.YachieA.,etal.(1999)JClinInvest.103:129–135. |
ProductImages
TypicalStandardCurvefortheHO-1ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-111.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.781–50ng/mL.
ProductCitations(1)
OtherCitations
Protectiveeffectofremotelimbpostconditioningviaupregulationofheme-oxygenase-1/BDNFpathwayinratmodelofcerebralischemicreperfusioninjury.
Ramagiri,S.andTaliyan,R.(2017)BrainRes.[Epubaheadofprint].
PubMedID:28535981ReactivityRatApplications:Brain
