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蚂蚁淘在线 / 品牌中心 / StressMarq / 应力MARQ/FCR1/SIH-180-1MG/1-mg

应力MARQ/FCR1/SIH-180-1MG/1-mg

价格
￥8500.00
货号：SIH-180-1MG
浏览量：127
品牌：StressMarq
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商品描述

Overview:

ProductNameFCR1
Description

FRETratiometricfluorescence-basedredoxsensor

MolecularFormulaC34H37N7O6
MolecularWeight639

Properties

StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
ProductTypeRedoxProbe
SolubilitySolubleinDMSO
SourceSynthetic
AppearanceOrangeSolid
SafetyPhrasesClassification:Caution:Substancenotyetfullytested.SafetyPhrases:S22-DonotbreathedustS24/25-AvoidcontactwithskinandeyesS36/37/39-Wearsuitableprotectiveclothing,glovesandeye/faceprotection
CiteThisProductFCR1(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SIH-180)

BIOLOGicalDescription

AlternativeNames7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzo[g]pteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide(FCR1)
ResearchAreasCancer,OxidativeStress
ScientificBackgroundFCR1orflavincoumarinredoxsensor1,isanovelratiometricfluorescentredoxsensorutilizedinfluorescencelifetimeimagingmicroscopyandflowcytometry.Intheoxidizedform,excitationofFCR1at405nmresultsinagreenfluorescencewithmaxemissionsat525nm.Treatmentwithamildreducingagent(includingsodiumcyanoborohydride,DTT,andglutathione)reducestheflavintherebydecreasingthegreenfluorescenceintensity,andincreasingtheblue.Re-oxidationcanbeachievedusingmildoxidizingagents.Ingeneral,theFCR1probecanbeusedtoobservechangesinoxidativecapacitywithoutinterferencefrombackgroundeffects.
References1.KaurA.,HaghighatbinM.A.,HoganC.F.,andNewE.J.(2015)Chem.Commun.Epub.

ProductImages

<p>Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

ChemicalstructureanddesignofFCR1(SIH-180),showingFRETprocessesinoxidisedform.Inset:photographsofcuvettesofFCR1inoxidisedandreducedformsunder365nmexcitation.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Fluorescence behavior of FCR1 (SIH-180) in the oxidized (green) and reduced (blue) forms, using 10 µM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

FluorescencebehaviorofFCR1(SIH-180)intheoxidized(green)andreduced(blue)forms,using10µM.Excitation:405nm.ReducedEmission:475nm.OxidizedEmission:520nm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Two photon – confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180, 10 µM, 15 min, λex = 820 nm) and (a) N-acetyl cysteine (50 µM, 30 min), (b) vehicle control and (c) H2O2 (50 µM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Twophoton–confocalmicroscopyimagingofHeLacellstreatedwithFCR1(SIH-180,10µM,15min,λex=820nm)and(a)N-acetylcysteine(50µM,30min),(b)vehiclecontroland(c)H2O2(50µM,30min)inblueandgreenchannels.Thepseudocolourratioimagesindicatetheratioofemissionintensityinthegreenchanneltobluechannel.Scalebarrepresents20µm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Fluorescence lifetimes of the donor (420 – 470 nm) in HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Fluorescencelifetimesofthedonor(420–470nm)inHeLacellstreatedwithFCR1(SIH-180,10µM,λex=820nm)andN-acetylcysteine,vehiclecontrolandH2O2.Pseudo-colourimagesrepresentmeanlifetime.Scalebarrepresents50µm.ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

<p>Flow cytometric studies of HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red). Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

FlowcytometricstudiesofHeLacellstreatedwithFCR1(SIH-180,10µM,λex=405nm)showingthefluorescenceratio(560/450nm)whentreatedwithN-acetylcysteine(blue),vehiclecontrol(green)andH2O2(red).ImagesusedwithpermissionfromKaurA,HaghighatbinMA,HoganCF,NewEJ.ChemCommun(Camb).2015Jun16;51(52):10510-3.

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StressMarq硫黄素T测定硫黄素T是一种荧光染料，可与富含β片层的结构（例如α突触核蛋白原纤维中的结构）结合。结合后，染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液（新鲜制备，并通过0.2μm注射器过滤器过滤）。在PBS pH 7.4中稀释硫黄素T，使每孔中硫黄素T的最终浓度为25μM（每孔体积= 100μL）。板：Lumox 96多孔板（Sarstedt目录号94.6000.024）。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体（或两者）添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值，需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱（600 rpm）中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置：温度：37°C读取类型：阱扫描波长：450 nm处的激发和485 nmPMT 处的发射增益：每次读取自动闪烁：6次摇动：读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。