StressMarq蚂蚁淘在线

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBSpH7.4,50%glycerol,0.025%Thimerosal
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	PeptideAffinityPurified
Clonality	Polyclonal
Specificity	Detects~34kDa.
CiteThisProduct	StressMarqBiosciencesCat#SPC-1121,RRID:AB_2710577
CertificateofAnalysis	A1:250dilutionofSPC-1121wassufficientfordetectionofDUSP2in10µgofHeLacelllysatebyECLimmunoblotanalysisusinggoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	Dualspecificityphosphatase2antibody,Dualspecificityproteinphosphatase2antibody,DualspecificityproteinphosphatasePAC1antibody,DualspecificityproteinphosphatasePAC-1antibody,DUS2_HUMANantibody,DUSP2antibody,DUSP2antibody,PAC1antibody,PAC1antibody,Serine/threoninespecificproteinphosphataseantibody
CellularLocalization	Nucleus
AccessionNumber	NP_004409
GeneID	1844
SwissProt	Q05923
ScientificBackground	DUSP2isamemberofthedualspecificityproteinphosphatasesubfamily.Thesephosphatasesinactivatetheirtargetkinasesbydephosphorylatingboththephosphoserine/threonineandphosphotyrosineresidues.Theynegativelyregulatemembersofthemitogen-activatedprotein(MAP)kinasesuperfamily(MAPK/ERK,SAPK/JNK,p38),whichisassociatedwithcellularproliferationanddifferentiation.DifferentmembersofthefamilyofdualspecificityphosphatasesshowdistinctsubstratespecificitiesforvariousMAPkinases,differenttissuedistributionandsubcellularlocalization,anddifferentmodesofinducibilityoftheirexpressionbyextracellularstimuli.

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APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

StressMarq硫黄素T测定硫黄素T是一种荧光染料，可与富含β片层的结构（例如α突触核蛋白原纤维中的结构）结合。结合后，染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液（新鲜制备，并通过0.2μm注射器过滤器过滤）。在PBS pH 7.4中稀释硫黄素T，使每孔中硫黄素T的最终浓度为25μM（每孔体积= 100μL）。板：Lumox 96多孔板（Sarstedt目录号94.6000.024）。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体（或两者）添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值，需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱（600 rpm）中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置：温度：37°C读取类型：阱扫描波长：450 nm处的激发和485 nmPMT 处的发射增益：每次读取自动闪烁：6次摇动：读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。

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