StressMarq蚂蚁淘在线

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	RabbitAntiserum,PBS,50%glycerol
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	Rabbitantiserum
Clonality	Polyclonal
Specificity	Detects~38kDa.Canrunupto45kDaonSDSPage.
CiteThisProduct	StressMarqBiosciencesCat#SPC-204,RRID:AB_2704181
CertificateofAnalysis	0.5µg/mlofSPC-204wassufficientfordetectionofAha1pin20µgofW303yeastlysatebycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	Activatorof90kDaheatshockproteinATPasehomolog1Antibody,Aha1Antibody,SHSA1Antibody,HSPC322Antibody,p38Antibody
ResearchAreas	Cancer,HeatShock,CellSignaling,ChaperoneProteins,ProteinTrafficking
CellularLocalization	Cytoplasm,EndoplasmicReticulum
AccessionNumber	NP_010500.3
GeneID	851800
SwissProt	Q12449
ScientificBackground	Aha1isamemberoftheHSP90cochaperonefamily,andisthoughttostimulateHSP90ATPaseactivitybycompetingwithp23andotherco-chaperonesforHSP90binding(1,2).ItmayaffectastepintheendoplasmicreticulumtoGolgitrafficking.Aha1alsointeractswithHSPCA/HSP90andwiththecytoplasmictailofthevesicularstomatistisvirusglycoproteins(VSVG)(3).Aha1isexpressedinnumeroustissues,includingthebrain,heart,skeletalmuscle,andkidney,andatlowlevels,theliverandplacenta.Aha1mightbeapotentialtherapeuticstrategytoincreasesensitivitytoHSPinhibitors(4).
References	1.HainzlO.,LapinaM.C.,BuchnerJ.,RichterK.(2009)JBiolChem.Epub. 2.HarstA.,LinH.,ObermannW.M.(2005)BiochemJ.387(pt.3):789-796. 3.LotzG.P.,BrychzyA.,HeinzS.,ObermannW.M.(2008)JCellSci.121(pt.5):717-723. 4.HolmesJ.L.,SharpS.Y.,HobbsS.,WorkmanP.(2008)CancerRes.68(4):1188-1197.

ProductImages

Currentlytherearenoimagesforthisproduct

ProductCitations(0)

Currentlytherearenocitationsforthisproduct.

APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

StressMarq硫黄素T测定硫黄素T是一种荧光染料，可与富含β片层的结构（例如α突触核蛋白原纤维中的结构）结合。结合后，染料的发射光谱发生红移并增加了荧光强度。以下方案用于使用α突触核蛋白预制的原纤维和单体生成硫黄素T测定法。准备dH2O中的1mM硫黄素T储备溶液（新鲜制备，并通过0.2μm注射器过滤器过滤）。在PBS pH 7.4中稀释硫黄素T，使每孔中硫黄素T的最终浓度为25μM（每孔体积= 100μL）。板：Lumox 96多孔板（Sarstedt目录号94.6000.024）。使用前在室温下解冻α-突触核蛋白等分试样。将10μM预制纤维或100μM单体（或两者）添加到适当的孔中。用移液器上下吸取混合液。浓度是估算值，需要进行优化以给出良好的信号而不会造成浪费。密封板并置于37°C的振荡培养箱（600 rpm）中。使用Softmax Pro软件6.5.1版在Molecular Devices Gemini XPS微孔板读取器上测量荧光。 XPS微孔板读取器设置：温度：37°C读取类型：阱扫描波长：450 nm处的激发和485 nmPMT 处的发射增益：每次读取自动闪烁：6次摇动：读取前20秒7.重新密封板并放入37°C的振荡培养箱中。8.定期从1到72小时获取读数。

资质认证

获得国家资质，权威认证！
全国联保

全国联保，官方无忧售后
正规发票

正规发票，放心购买
签订合同

签订合同，保障您的权益

/**/