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蚂蚁淘在线 / 品牌中心 / StressMarq / StressMarq/StressXpress® 20S Proteasome Activity Kit/SKT-133-96/96-well

StressMarq/StressXpress® 20S Proteasome Activity Kit/SKT-133-96/96-well

价格
￥7500.00
货号：SKT-133-96
浏览量：127
品牌：StressMarq
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商品描述

Overview:

ProductName20SProteasomeActivityKit
Description

Fluorometricdetectionofpurified20Sproteasomeactivity

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesPurifiedProteins
DetectionMethodFluorometricAssay
AssayTypeContinuousKineticAssay
UtilityActivitykitusedtoscreenpotentialproteasomeinhibitorsandactivators,andtomeasureandquantify20Sproteasomeactivityincontinuouskineticandend-pointassays.
IncubationTime15-30minutes
NumberofSamples40samplesinduplicateusingtheplateformat,or100x50µLmicro-cuvettebasedassays
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeActivityKits
AssayOverviewThe20SProteasomeActivitykitfacilitatestherapid,robustmeasurementofproteasomeactivityinvitro.Thekitutiliseshighpurity,fluorogenicsubstrateSuc-LLVY-AMCtogetherwithsuitablecalibrationstandardsandcontrolsfortheaccurateandsensitiveassessmentofproteasomechymotrypsin-like(β5)activity.Continuouskineticorend-pointassayscanbeperformedinmicro-cuvettesorin96-wellplateformatformulti-sampleanalysis.Containssufficientmaterialsforonefull96-wellplateassayorupto100x50µLmicro-cuvettebasedassaystoberun.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-133A10xStressXpress®proteasomeassaybuffer1ml(reconstituted)
SKC-133B20Sproteasome10µg
SKC-133CMG132(controlinhibitor)100µg
SKC-133DStressXpress®96wellassayplate1plate
SKC-133EStressXpress®AMCstandard1.75µg
SKC-133FSuc-LLVY-AMC(chymotrypsin-likesubstrate)500µg
CiteThisProduct20SProteasomeActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-133)

BIOLOGicalDescription

AlternativeNamesChymotrypsin-like(β5)ActivityKit
ResearchAreasCancer,Apoptosis,CardiovascularSystem,CellSignaling,Neurodegeneration,Neuroscience,Post-translationalModifications
ScientificBackgroundProteasomesarenon-lysosomalproteolyticcomplexeslocalisedprimarilyinthecytoplasmandinthenucleusofeukaryoticcells.TheyareresponsIBLefortheubiquitin-mediateddegradationofshorthalf-lifeproteinsandpeptidesthatareinvolvedinessentialcellularprocessesincludingcell-cycleregulation,apoptosisandtranscriptionalregulation,innateimmunityandantigenprocessing,andintheremovalofredundantordamagedproteins.Assuchproteindegradationbytheubiquitin-proteasomepathwayhasamajorregulatoryfunctionforproliferationactivityandsurvivalofbothnormalandmalignantcells,anditsdysfunctionhasbeenimplicatedinawiderangeofotherdiseaseprocessesincludingneurodegenerative,cardiovascularandmetabolicdisorders.The26Sproteasomestructureiscomposedofa20Sproteasomecatalyticcorecomplexandoneortwo19S(PA700)regulatorysubcomplexes.The20Scorecomprisestwocopiesof14subunits(7alphasubunitsand7betasubunits)arrangedinaα7β7β7α7cylindricalarray.Proteolyticactivitiesaredeterminedbytheβ1(caspase-like),β2(trypsin-like)andβ5(chymotrypsin-like)subunits,accesstowhichisguardedbytheα-subunits.The19SregulatoryunitconsistsofsixATPaseandatleasttennon-ATPasesubunitsthatarerequiredforubiquitinatedproteinbinding,deubiquitination,substrateunfoldingandtranslocationtothe20Scatalyticcore.Varyingcatalyticsubunitcomposition(β1,β1i;β2,β2i;β5,β5i)resultsinavarietyofpossiblesubtypesfromfullconstitutiveproteasome(β1,β2,β5)throughmixedpopulationstofullinducible/immunoproteasome(β1i,β2i,β5i).AlternativeregulatorycomplexessuchasthePA200and11Sproteasomeactivatorsconferdifferentsubstratespecificitiesandactivitycomparedtothe19Sregulator.
References1.Saeki,Y.&Tanaka,K.Methodsinmolecularbiology(Clifton,N.J.)832,315–37(2012).
2.Frankland-Searby,S.&Bhaumik,S.R.Biochimicaetbiophysicaacta1825,64–76(2012).
3.Basler,M.,Kirk,C.J.&Groettrup,M.Currentopinioninimmunology1–7(2012).
4.Löw,P.Generalandcomparativeendocrinology172,39–43(2011).
5.Dennissen,F.J.a,Kholod,N.&VanLeeuwen,F.W.Progressinneurobiology96,190–207(2012).
6.Geng,F.,Wenzel,S.&Tansey,W.P.Annualreviewofbiochemistry81,177–201(2012).

ProductImages

<p>AMC Standard Curve for 20S Proteasome Activity Kit. Representative plot of fluorescence measurements (RFU) of serial dilutions of AMC Standard at recommended concentrations.</p>

AMCStandardCurvefor20SProteasomeActivityKit.Representativeplotoffluorescencemeasurements(RFU)ofserialdilutionsofAMCStandardatrecommendedconcentrations.

<p>Continuous kinetic enzyme activity assay showing the time course of control 20S proteasome activity with Suc-LLVY-AMC substrate using the StressXpress® 20S Proteasome Activity Kit. 20S proteasome (~2.5nM) was incubated with 100µM Suc-LLVY-AMC in proteasome assay buffer for 30 minutes at room temperature alongside a substrate only (blank) control reaction. Fluorescence measurements (RFU) were taken at 30 second intervals and plotted vs. time.</p>

Continuouskineticenzymeactivityassayshowingthetimecourseofcontrol20SproteasomeactivitywithSuc-LLVY-AMCsubstrateusingtheStressXpress®20SProteasomeActivityKit.20Sproteasome(~2.5nM)wasincubatedwith100µMSuc-LLVY-AMCinproteasomeassaybufferfor30minutesatroomtemperaturealongsideasubstrateonly(blank)controlreaction.Fluorescencemeasurements(RFU)weretakenat30secondintervalsandplottedvs.time.

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