Clone/AB feature:

(#MAB0875)

Antibody generation:

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant 4-1BB Ligand extracellular domain. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.

Antigen:

BALB/c mouse B lymphoma cell line

Stability:

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

Reconstitution:

Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Description:

4-1BB ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa Nterminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but shares only low aa sequence homology (14-16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart. 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases. By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as noncovalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 41BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and upregulates expression of B71 and B72 costimulatory molecules. RELIATech为在昆虫细胞（杆状病毒）和大肠杆菌中表达重组蛋白提供全面的客户服务。这些服务分为一系列步骤，可以单独执行或组合执行，以便您可以选择实际需要的步骤。这些服务旨在充分满足您的大多数需求，从将基因克隆到杆状病毒转移载体或大肠杆菌表达载体，重组杆状病毒或大肠杆菌菌株的产生到重组蛋白的生产和纯化。如果需要，我们还将尝试建立一种测定纯化蛋白的生物学活性的方法。