StABIlity:

ThelyophilizedhumanFGF-9,thoughstableatroomtemperature,isbeststoreddesiccatedbelow0°C.ReconstitutedhumanFGF-9shouldbestoredat-20°Cto-70°C.Repeatedfreeze/thawcycleswillresultinsignificantlossofactivity.

Reconstitution:

Forreconstitutionitisrecommendedtoaddaceticacid(50mM)containingatleast0.1%BSA/HSA(orFCS)toprepareastocksolutionofnolessthan100mg/ml.Fordilutionitisrecommendedtousesterilephosphate-bufferedsalinecontainingatleast0.1%BSA/HSAorusingmediumwithFCSfordilution.

Description:

TheFGFfamilyiscomposedofatleast22polypeptidesthatshowavarietyofBIOLOGicalactivitiestowardscellsofmesenchymal,neuronalandepithelialorigin.Allmembersareheparin-bindinggrowthfactors.AllFGFshavetwoconservedcysteineresiduesandshare30-50%sequenceidentityattheaminoacidlevel.FGF-9,alsonamedglia-activatingfactor,wasoriginallyidentifiedandpurifiedfromthesupernatantofahumangliomacelllineasamitogenforglialcells.FGF-9hasalsobeenshowntostimulatetheproliferationofoligodendrocytestype2astrocyteProgenitorcells,PC12cellsandBalb/c3T3cells.UnlikeacidicandbasicFGF,theFGF-9moleculeisnotamitogenforhumanvascularendothelialcells.FromthefourFGFreceptorsknown,FGF-9bindsonlytotheFGF-R3.ConstitutiveactiveFGF-R3inhumanisconnectedwithanormalbonedevelopment.ThehumanFGF-9CDNAencodesa208aminoacidresidueproteinthatcontainsasingle,potentialN-linkedglycosylationsite.Thenativeproteinisglycosylatedandisefficientlysecretedaftersynthesis,althoughFGF-9lacksatypicalsecretionsignal.RatandmouseFGF-9showaveryhighhomologytohumanFGF-9.ThetranscriptsforFGF-9havebeenfoundinbrainandinkidneytissue. RELIATech为在昆虫细胞（杆状病毒）和大肠杆菌中表达重组蛋白提供全面的客户服务。这些服务分为一系列步骤，可以单独执行或组合执行，以便您可以选择实际需要的步骤。这些服务旨在充分满足您的大多数需求，从将基因克隆到杆状病毒转移载体或大肠杆菌表达载体，重组杆状病毒或大肠杆菌菌株的产生到重组蛋白的生产和纯化。如果需要，我们还将尝试建立一种测定纯化蛋白的生物学活性的方法。