ORDERINGINFORMATION

CatalogNo.Size

18842F100ug

Format:1mg/mlconjugatedtofluoresceinisothiocyanate(FITC)inPhosphateBufferedSaline(PBS),pH7.2,0.2%BSA,0.09%sodiumazide.

BACKGROUND

AGST-tagisoftenusedtoseparateandpurifyproteinsthatcontaintheGST-fusionprotein.Thetagis220aminoacidswhich,comparedtotagssuchasthemyc-tag,isquitelarge.ItcanbefusedtoeithertheN-terminusorC-terminusofaprotein.ManycommerciallyavailablesourcesofGST-taggedplasmidsincludeathrombindomainforcleavageoftheGSTtagduringproteinpurification.

SPECIFICATIONSUMMARY

Antigen:Glutathione-S-Transferase(GST)fromSchistosomajaponicum.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonGST.

APPLICATIONS

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunofluorescence:useatadilutionof1:100-1:400.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.Shieldfromlight.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.