ORDERINGINFORMATION

CatalogNo.:13042

Format:100ugantigen-affinitypurifiedIgGinTris-citrate/phosphatebuffer,pH7-8,0.09%sodiumazide.

BACKGROUND

AurorakinaseA,alsoknownasserine/threonine-proteinkinase6,isanenzymethatinhumansisencodedbytheAURKAgene.AuroraAisamemberofafamilyofmitoticserine/threoninekinasesassociatedwithimportantprocessesduringmitosisandmeiosis.AuroraAisactivatedbyoneormorephosphorylations,anditsactivitypeaksduringtheG2phasetoMphasetransitioninthecellcycle.AuroraAdysregulationhasbeenassociatedwithhighoccurrenceofcancer.

SPECIFICATIONSUMMARY

Antigen:Syntheticpeptiderepresentingaportionoftheproteinencodedwithinexons5and6.

Accessionno.:NP_003591.2

GeneID:6790

HostSpecies:Rabbit

Specificity:ThisantibodyreactswithhumanAurorakinaseA,andtheepitoperecognizedmapstoaregionbetweenresidues75-125ofhumanAurorakinaseA.Basedon100%sequenceidentitythisantibodyispredictedtoreactwithorangutan,gorilla,chimpanzee,andNorthernwhite-cheekedgibbon.

APPLICATIONS

Immunoblotting:1:5,000-1:20,000dilution

DetectionofhumanAuroraAinHeLaandHEPG2wholecelllysateswithcatalogno.13042at0.2ug/ml.Detectionbychemiluminescence.

Immunoprecipitation:1-5ug/mglysate.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableat2-8°Cforone(1)yearfromdateofreceipt.

Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.