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CatalogNo.:56214

Size:100ulpurifiedantibodyinPBS,pH7.4

Antigen:GSTfusionproteincorrespondingtofull-lengthRad51(aa1-338)ofhumanRad51expressedin

E.coli.

HostSpecies:MouseAntibodyClass:IgG2b/κStABIlizers:None

Preservatives:None.Availableonrequest.

ThisantibodyrecognizeshumanRad51,a37kDproteinthatinteractswithBRCA1.Thisinteractionisimportantfortumorsuppression.BRCA2andRad51havealsobeenshowntointeractviatheBRCrepeatslocatedwithexon11ofBRCA2.Whentheserepeatsaredeleted,theinteractionislost,andcellsbecomehypersensitivetoDNAdamagecausedbymethylmethanesulfonate(MMS).

Immunoblotting,immunoprecipitation:useat1-5ug/ml.

Positivecontrol:T24bladdercarcinomacellsandrecombinantfusionprotein.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.

Shinohara,Aetal.1993.NatureGenetics4:239-243;Scully,Retal.1997.Cell88:265-275;Chen,PLetal.

1998.PNAS(USA)95:5287-5292.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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