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- AntiHuman Caspase10 (CT)
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Anti-CAD(CT)Antibody
CatalogNo.:2416
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.ThesedeathsignalsfinallycausethedegradationofchromosomalDNAbyactivatedDNase.AmouseDNasethatcausesDNAfragmentationwasidentifiedrecentlyanddesignatedCAD(caspaseactivateddeoxyribo-nuclease).ActivationofCAD/DFF40,whichcausesDNAdegradation,isthehallmarkofapoptoticcelldeath.
Antigen:Peptidecorrespondingtoaa205-222ofmouseCAD(accessionno.O54788).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthmouseCAD(40kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat2ug/ml.
WesternblotanalysisofCADinmousekidneytissuelysatewithCADantibodyat2μg/ml.
Immunohistochemistry:useat2ug/ml.
ImmunohistochemicalstainingofCADinmousekidneytissuewithCADantibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:Mouselungtissuelysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
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