E. coli K99 Pilus Monoclonal Antibodies
Catalog No.
18401
Clone No.
K9911-201.3
MAb Subtype
IgG1
Size
100ug, 500ug
Library Pack No.
184101
100ug/clone
All 4 clones
18402
K9912-349.2
IgG1
100ug, 500ug
18403
K9912-418.2
IgG1
100ug, 500ug
18404
K9912-444.5
IgG1
100ug, 500ug
Catalog No.
18401
Clone No.
K9911-201.3
MAb Subtype
IgG1
Size
100ug, 500ug
Library Pack No.
184101
100ug/clone
All 4 clones
18402
K9912-349.2
IgG1
100ug, 500ug
18403
K9912-418.2
IgG1
100ug, 500ug
18404
K9912-444.5
IgG1
100ug, 500ug
ORDERING INFORMATION
Format: Protein G-purified antibody in PBS, pH 7.4.
BACKGROUND
Bovine enteric colibacillosis is an economically important disease in neonatal calves caused by enterotoxigenic E. coli (ETEC) which adhere to intestinal cells using pili. Most ETEC isolated from cattle use K99 pili to bind to enterocytes of the small intestine. K99 pili are primarily composed of a repeating 18.2kDa major subunit which is responsible for binding to host glycoprotein receptor, N-glycolylneuraminic acid-GM3. A 7.1kb operon of eight genes on a plasmid encode the pili and other associated proteins responsible for regulation, transport, and assembly of K99 pili.
SPECIFICATION SUMMARY
Antigen: Purified E. coli K99 (B44) pili.
Host Species: Mouse
Specificity: These antibodies recognize K99+ E. coli. They do not cross-react with K99- negative E. coli (B41), E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.
APPLICATIONS
These antibodies have been qualified for use in ELISA to detect K99+ E. coli.
DILUTION INSTRUCTIONS
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20oC to -70oC. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.
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