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Lipopolysaccharides(LPS),alsoknownaslipoglyansandendotoxins,arelargemoleculesconsistingofalipidandapolysaccharidecomposedofO-antigenmoutercoreandinnercorejoinedbyacovalentbond.TheyarefoundintheoutermembraneofGram-negativebacteriaandelicitstrongimmuneresponsesinnormalmammaliancells.LPSfromE.coliisparticularlypotentinsystemicinflammationandhavelongbeenrecongnizedasakeyfactorinsepticshockandalsousedinresearchtoinducesynthesisandsecretionofgrowthpromotingfactorssuchasinterleukins.
MDBiosciencesprovideslyophilizedlipopolysaccharide(LPS)forinvivoandinvitrouse.TheLPSisisolatedfromEscherichiacolibyamodificationofthephenolextractionmethodofWestphalandJann.ThismaterialhasbeenextractedfrombacterialcellswallsofE.coli055:B5.Theendotoxinhasbeenfutherpurifiedtoremovetracesofresidualproteinandnucleicacids,producinghighlypurifiedLPS.TheLPSisessentiallyfreeofnucleicacidandproteinandischemicallycharacterizedwithrespecttotheirphosphateandKDO(2-keto-3-deoxyoctonate)contents.ThehighlypurifiedLPShasbeenthenre-extractedbythemethodofMantheyandVogeltoeliminateresidualproteincontamination.
Application:Forin-vivo/in-vitrostudies.
Storage:Keeptightlyclosed.Storeat2-8°C.
