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蚂蚁淘在线 / 品牌中心 / MD Bioproducts / MD生物制品/神经冷冻/M036041/10毫升或30毫升

MD生物制品/神经冷冻/M036041/10毫升或30毫升

价格
面议
货号：M036041
浏览量：127
品牌：MD Bioproducts
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商品描述
Overview: 

TheNeuroFreezekitcanbeusedtofreezeprimaryneuronsfromE18/19ratandP0/P1mousehippocampiandcortices.NeuronsfrozeninthismediamaintainhighlevelsofviABIlity(upto90%)uponthawing.Theseneuronsshowalltheexpectedcharacteristicsofprimaryneurons,includingappropriatemorphologyandexpressionofneuronalandsynapticMarkers.Theavailabilityofthismediawillenableinvestigatorstofreezeprimaryneuronsfromembryonicrats(E18)orP0/P1wildtypeorgeneticallyengineeredmice.

Data/Specifications: 

Species: mouse,rat

 

 

 

How_To_Use:

FreezingofPrimaryNeurons:    

  1. Obtaindissociatedprimaryneuronsfromrat/mousehippocampus/cortexasdescribed(Beaudoinetal).Re-sUSPenddissociatedcellsinminimalvolumeofplatingmedia.
  2. EstimatecellviabilityonaHemocytometer.
  3. Donotexceedamaximumof4millioncells/vialforfreezing.Itisrecommendedtofreeze1to4millioncellspervial.
  4. Re-suspendcellstobefrozenin900μlofReagentA.
  5. Add10%(100μl for1mL)ofReagentBtothevial.
  6. Add1%(10μlfor1mL)ofReagentCtothevial.
  7. Gentlymixandplacethevialinisopropanol bathin-80°Cfreezer.
  8. Nextday,placevial(s)in liquidnitrogen.

THAWINGPROTOCOL

  1. Followyourlaboratory’sprotocolforcoatingsubstrate.
  2. Addpre‐maintenancemedia(ReagentD)todishes/platesandincubatein37°Cincubatorforaminimumof2hours(recommended4hours).
  3. Bringavialofcellsfromliquidnitrogenandquicklythawin37°Cwaterbath(usuallytakesabout90seconds)–itiscriticalthatthawingisaccomplishedasfastaspossIBLe.
  4. Estimatecellviability.
  5. Seedcellsontothesubstrate(SeePlatingDensityChartbelow)
  6. Aftercellshaveattachedtothesubstrate(3-4hours),replacepre-maintenancemediawithyourlab’sneuronmaintenancemedia(B-27containingmaintenancemediaisrecommended).
  7. MaintainneuronsinB27containingmaintenancemediaat37°C.

 

MD Bioproducts的ELISA法目录编号名称数量/尺寸M036005N小鼠OVA-IgE ELISA96口井M046006酶联免疫吸附测定96口井M046008Aggrecanase活性ELISA96口井M046009Aggrecanase活性，灵敏的ELISA96口井M046010降钙素ELISA96口井M056011内啡肽（β-Endorphin）ELISA96口井M046013EPO ELISA96口井M046043HtrA丝氨酸肽酶-1（HtrA1）ELISA，人类96口井M056014亮氨酸-脑啡肽酶联免疫吸附测定96口井M046015MMP-13（活化）ELISA96口井M046016PTH，完整ELISA96口井M056018P物质ELISA96口井