LifeSensors/SI200-Panel Customized Ubiquitin Chain Kit, 5µg of each (K6, K11, K27, K29, K33, K48, K63 and linear)/SI200-Panel

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¥8000.00
货号:SI200-PanelCustomizedUbiquitinChainKit,5µgofeach(K6,
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品牌:Life Sensors
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商品描述
ThereversIBLeconjugationofubiquitinandubiquitin-likeproteinstosubstratesregulatesalargenumberofcellularprocesses.Decipheringthesecomplexpathwaysisthefocusofmanyresearchersbuttheworkhasbeenslowedbythelackofhighqualityreagents.Deubiquitinatingenzymes(DUBs)recognizeandcleavetheisopeptidebondbetweenubiquitinmoietiesorbetweenubiquitin andthetargetprotein.MeasurementoftheactivityoftheseenzymesforkineticstudyorinhibitorscreeninghasbeentrADItionallycarriedoutusingubiquitinconjugatedtofluorescentleavinggroups(AMCorRhodamine)attheC-terminus.Whilethesearesuitablesubstratesformanydeubiquitinatingenzymes,thecleavageoftheamidebondservesasaproxyforthetrueactivityofisopeptidases,i.e.cleavageofanisopeptidebond.

LifeSensorshasproducedacompletepanelofalleightpossibledi-ubiquitinmolecules(includingCtoN-linkedlinearmolecules)fordeterminingthelinkagespecificactivityofindividualDUBs. Thiskitisaconvenientcollectionofthesedi-Ubmoleculesfortheaninitialsurveyoflinkage-specificity.


Info

Contents5μgamountsofeachofthefollowingdi-ubiquitins:linear,K6,K11,K27,K29,K33,K48,andK63
Purity>=95%(RPHPLC)
PhysicalStateLiquid(0.05%HOAc),lyophilizedpowder
Storage-80°C. Avoidrepeatedfreeze/thawcycles


Applications

  • Determinethelinkage-specificactivityofindividualde-ubiquitnatingenzymes(DUBs).

Documents

Lifesensors 是一家生物技术公司,致力于为治疗,药物发现和研究市场开发支持技术。通过提供创新的研究工具,确立了在泛素和类泛素蛋白途径中的先进地位。此外,我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用