BasedonUBAdomainsknowntopossessahighaffinityforubiquitin,TandemUbiquitinBindingEntities(TUBEs)havebeendevelopedfortheisolationandidentificationofubiquitinatedproteins.TUBEsdisplayuptoa1000-foldincreaseinaffinityforpolyubiquitinmoietiesoverthesingleubiquitinbindingassociateddomain(UBA).Inaddition,TUBEshavebeendemonstratedtoprotectpolyubiquitinatedproteinsfrombothdeubiquitinationandproteasome-mediateddegradation,allowingforthedetectionofrelativelylowabundantproteinsthatcannotbedetectedwithcurrenttechnologies. 

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Info

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AffinityTag	GST
MolecularWeight	56kDa

Applications

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• Pulldownofpoly-ubiquitylatedproteinsfromcellandtissuelysates
• Protectionofpoly-ubiquitylatedproteinsfrombothdeubiquitylationanddegradationbytheproteasome

Benefits

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• Upto1000-foldhigheraffinitythanthesingleUBAdomain
• Avoidoverexpressionofaffinity-taggedubiquitinsfor pulldowns
• Protectpoly-ubiquitinchainswithoutinhibitorsspecifictotheproteasomeordeubiquitylaseactivity
• Replace ubiquitinspecific antibodiesforenrichmentofpoly-ubiquitylatedproteins

Documents

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• Datasheet
• Manual
• LimitedUseLabelLicense
  

Lifesensors 是一家生物技术公司，致力于为治疗，药物发现和研究市场开发支持技术。通过提供创新的研究工具，确立了在泛素和类泛素蛋白途径中的先进地位。此外，我们的原核和真核蛋白表达平台 SUMOpro 和 SUMOstar 在世界范围内被学术、政府、制药和生物技术实体所使用