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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
Foruse in “pre-clearing” cell lysates, as well as a negative control for thepulldown of polyubiquitylated proteins in conjunction with magnetic beads-conjugatedTUBEs (UM401M and UM402M).
Generalrecommendations: Pre-clear or use as a negative control for 1ml of cell lysate(3-5 mg/ml total protein) through incubation with 100µl of slurry for 1hr(4°C). Individual conditions may have to be optimized by the end user. Controlmagnetic beads is generated by blocking an activated matrix (identical to thatused for TUBEs conjugation) with ethanolamine. Control magnetic beads (1 mL) issupplied as a slurry in PBS, pH 7.2.
Info
Storage: +4°C. Storage below this temperature may result in freezing and/or loss of function. |
Applications
- Negative control for pull-down experiments
- "Pre-clearing" of cell lysates prior to pull-down experiments
Documents
- Documents
