Specifications

Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is refered to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one (of seven) lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation.This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines. This molecule will not participate in polyubiquitin formation, and can therefore be used in control experiments for the study of ubiquitylation.Info

Source	Human recombinant
Form	liquid
Molecular weight	8,760 Da
Purity	>=95% by SDS-PAGE
Affinity Tag	None
Concentration	4 mg/mL
Buffer	20 mM Tris, pH 8.0, 0.15 M NaCl, 10% glycerol
Storage	-80°C, avoid cycles of freeze/thaw
Stability	1 year under recommended storage conditions

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