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Specifications
IQFDiUbTechnologyLifeSensors’diubiquitinsubstratesrepresentanewclassofsubstratesforthecontinuousfluorescentmeasurementoftrueisopeptidaseactivity.TheC-terminusofwild-typeubiquitinisconjugatedviaanisopeptidebondtolysine11,48,or63ofasecondubiquitinmoleculewiththeresultantdiubiquitinforminganinternallyquenchedfluorescentFRETpair(IQF).Eachubiquitinislabeledwithasinglemoleculeofeitherafluorescentreporter(i.e.TAMRA)orahighlyefficientquenchingdye.CleavageoftheIQFDiUbbydeubiquitylasesleadstoseparationofthefluorophorefromquencherandsubsequentincreaseinobservedfluorescence.Theubiquitin-TAMRAreferencestandardcanbeusedtooptimizeandcalibratetheperformanceofindividualplatereaders(orfluorometers)formeasurementoftheratesofhydrolysisofourIQF-DiUbbyDUBs.
Info
| Amount | 25µg |
| MW | 9,161Da |
| Concentration | 40µM |
| Purity | >=90% |
| Buffer | 50mMSodiumMES,pH6.0 |


