Specifications

Todate,detectingchangesintheubiquitylationofspecificsubstrateproteinsinresponsetoexternalstimuli,e.g.stress,cytokineexposure,drugcandidatetreatment,etc.,hasbeenalong,labor-intensiveprocessinvolvingimmunoprecipitationfollowedbygelelectrophoresisandWesternblotanalysis. Thismethodislowthrough-put,resourceintensive,andonlysemi-quantitativeatbest. WehavedevelopedtheUbiQuant™Skitasafacile,robust,andquantitativealternativetoIP/WBanalysis.BuiltonLifeSensorsUbiQuantplatform,thesystemcapturesubiquitylatedproteinsinthewellsofamicrotiterplateandthenusesanantibodytotheproteinofinterest(eithernativeortagged)toquantitatetheamountoftheproteinbound.

Note:Ifusingatagged-substrate,theusermustspecifytheepitopetaginordertoreceivetheappropriateantibodyfordetection(e.g.myc,HA,FLAG^®,V5,GST,etc. Unfortunately,His₆cannotbeusedforthisassay.)

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