For isolation of CD45RA+ T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer® components for positive cell isolation with magnetic microbeads*and for removal of Streptamers from isolated cells.

1.) Strep-Tactin® Magnetic Microbeads for Fab Streptamers; 2 vials for 5x10^8 cells each

2.) CD45RA Fab-Strep, human for isolation of T-cells; 2 vials for 5x10^8 cells each

3.) D-Biotin stock solution for Streptamer® technology; 2x 1 ml

4.) Buffer IS, Streptamer® Washing Buffer, 10x concentrated; 50 ml

This product requires dry ice shipment and is shipped directly from our Headquarters in Germany.

*Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 ml and 2 x 50 ml Falcon tubes.

Principle of Selection:

Isolation of human CD45RA+ T cells is performed by positive selection. PBMCs are labeled with magnetic CD45RA Fab Streptamers®. Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet. This CD45RA positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position). The magnetic Streptamers migrate toward the tube wall and the label-free cells remain in the supernatant. Biotin is removed by washing.

The resulting cell preparation is highly enriched with CD45RA+T cells with a purity of more than 90%. No depletion steps and no columns are needed.

IBA lifesciences公司总部位於德国，致力於生命科学之研究，以提供高质量的产品及服务为使命。二十年来，以杰出的专利技术Strep-tag®系统为基础，IBA发展出完整的产品组合，涵盖重组蛋白的表达丶纯化与固定以及特定细胞从全血或相关体液中分离的技术，广泛的被应用於学术及商业研究机构中。至今在美国已设置了配送中心，而全球有超过40家的代理商，客户遍布全世界。同时，我们也是全球领先的核酸合成专家，在DNA及RNA寡核苷酸的合成上已有超过二十年的经验，尤其能根据客户的选择，生产含有特定染料或化学修饰的特殊寡核苷酸。知名的Strep-tag®/Strep-Tactin®产品系列，适合用来纯化高纯度及高活性的蛋白质。而最新的突破性产品－更具亲和力但仍具可逆性的Strep-Tactin® XT－更进一步的扩展了Strep-tag®的应用领域，例如检测蛋白质相互作用或需要更稳定蛋白质链结的检测。另一专利科技—Streptamer® 技术，则被应用在磁性或非磁性细胞分离法上，其试剂的可逆性使分离後的细胞保有完整的功能及活性。另一应用则是结合此系列中含萤光染料的产品，将功能性免疫细胞染色。IBA的核酸合成部门则根据客户的要求，提供个别的订制服务，合成萤光标记或其他特殊标记的DNA或RNA寡核苷酸。近年来日趋热门的适体技术(Aptamer)，亦是我们的专业核酸合成服务重点之一。IBA lifesciences是生命科学领域的杰出供应商，我们的产品组合提供了研究人员在各种不同领域里的解决方案。专业的产品开发部门及技术支援服务让我们能与客户紧密合作，促进生命科学研究的发展。更详细的产品信息，欢迎参观我们的官网www.iba-lifesciences.com。