- 外显子-DNA-PS：从血浆和血清中分离循环和EV相关DNA
- 生物化学
- Exo准备
- 细胞增殖和分离分析
- 外显子DNAc PS：从血浆和血清中分离循环和EV相关DNA
- 尿液中冻干纯化外体
- 外显子DNAc-UC：尿液及细胞培养液中循环及EV相关DNA的分离
- 外显子-DNA-UC:尿液和细胞培养液中循环和EV相关DNA的分离
- 单克隆抗体
- 外显子-DNA-UC:尿液和细胞培养液中循环和EV相关DNA的分离
- 外显子DNAc-UC：尿液及细胞培养液中循环及EV相关DNA的分离
- Exo试验：即用ELISA定量试剂盒
- RNA碱性试剂盒
- 冻干纯化血浆外体
- 氧化应激
- 冻干纯化血清外体
- 唾液外体的冻干纯化
- ELISA免疫平板
- 不同细胞系外体的冻干纯化
- 纯EVs：大小排阻色谱柱
- 肿瘤总RNA：肿瘤外体免疫捕获和RNA提取试剂盒
Immunoplates for Overall Exosome capture from human saliva.
|
Product |
White 5 standard 96-well format pre-coated plates |
|
Sample type |
Human saliva |
|
Exosome capiture |
Overall exosome |
|
Starting material |
Up to 100 µL of biological sample can be loaded per well. Unfractionated human saliva sample can be directly used for capture. |
|
Product overview |
Our multiwell plates are standard 96-well format where assays can be conducted as singletons and/or multiple wells. This enables optimization of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). |
|
Antibody used for coating |
Polyclonal rabbit antibodies |
|
Coating |
Covalent |
|
Packaging |
The plate is packaged in an opaque aluminum pouch which complies to food and pharmaceutical regulations. Easy to open and re-sealable by zip-lock feature. |
|
Shipment and storage |
Immunoplates are shipped at 4°C and storage conditions as recommended in the product insert. |
|
Expiration date |
Unopened: 2 year stored at 4°C Opened: 6 month stored at 4°C |
Applications:
Proteomic analyses
Exosomes incorporate a wide range of membranes and cytosolic proteins involved in many cellular functions and reflecting both their endosomal origin and a protein fingerprint unique for parent cell type and condition. HBM Immunoplates are an useful platform for Exosome capture from different biological sources and allow quantification through ELISA technique of protein exosomal markers.
Work with exosomal RNA
Exosomes shuttle RNA molecules (mRNAs and miRNAs), both classes proved to be functional in target cell, being translated or modulating gene silencing, respectively. Overall, exosome molecular composition distinguishes resting from activated, healthy from diseased cells.
RNA extraction could be performed after capturing exosomes on the plate. Recommended for RNA extraction: HansaBioMed Exosome Total RNA Extraction Kit.
Total exosome quantification
Immunoplates are part of the Ready to Use Kit for capture and quantification. The kit contains all the necessary components exosome quantification.
Related products:
Exosome standards
RNA extraction kits
Quantification kits
HansaBioMed LLC是一家完全致力于外泌体（exosomes）研究工具开发的欧洲公司。公司以“为生命科学领域外泌体研究开发和商业化相关专利技术、试剂盒以及相关设备”为使命。HansaBioMed的研发生产基地位于爱沙尼亚首都塔林（Tallinn）科技园和意大利锡纳 (Siena)。 HansaBioMed公司可为全球生命科学市场提供最广泛的外泌体研究工具，是历史最悠久也是唯一一家只专注于提供高品质外泌体分离与分析相关产品的公司。目前公司主要提供的产品包括外泌体标准品（Exosome Standards）、外泌体结合抗体（Exosome binding Antibodies）以及特异性外泌体亚群富集试剂盒等。同时，HansaBiomed公司与来自学术界和产业界的国际知名研究团体合作，进一步发展其技术平台以及独有的生物标志物产品线。学术合作单位包括罗马的Istituto Superiore di Sanità研究所、美国哈佛大学和耶鲁大学以及里加的拉脱维亚大学等。
