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ProductHighlights:
TheTruSightHLAv2SequencingPanelprovidesclinicalresearcherswithabroad-coverage,ultra-high–resolutionhumanleukocyteantigen(HLA)typingsolutionforsimple,rapidassessmentoftheHLAregioninasingleassay.Itfeatures:
- High-resolutionsequencingof11HLAloci
- Workflowwithlibrarypreparation,sequencinganddataanalysis/reporting
- Accurateandhigh-resolutionHLAtypingforunambiguousresults
CaptureFullHLAGeneSequences
TruSightHLAv2coversallcommonlytypedHLAloci,plusthosewithemergingrelevance.ThispanelenablessequencingofClassIHLA-A,B,andC;ClassIIHLA-DRB1/3/4/5,HLA-DQA1,HLA-DQB1,HLA-DPA1,andHLA-DPB1.Thisexpandscoveragebeyondtheclassicloci,withfullgenecoverage,enablingdiscoveryofnewalleleswithouttheneedtodesignnewprimers.
Sample-to-ReportWorkflowwithOptimizedDataAnalysis
ThecomprehensivesolutionincludesreagentsandsoftwareoptimizedforHLAanalysis.Asimplifiedworkflowenablesturnaroundtimeoflessthan48hoursandincreasedefficiencywithlessthan4.5hoursofhands-ontime.On-instrumentsoftwareanalyzessequencedata;TruSightHLAAssignSoftwareprovidesflexIBLeoptionsforpost-analysisreportingofresults.
High-AccuracyHLATyping
TheexpandedcoverageofTruSightHLAv2provideshighresolution,eliminatingtheneedforfollow-uptestingtoobtainaconfidenttypingresult.ViewHLALoci.
HowTruSightHLAv2Works
TruSightHLAv2amplifiesHLAlociwithlong-rangePCR.Afteramplification,NexteratagmentationfragmentstheDNAandleavesbehindasequencetag.AnadditionalPCRthenaddssequenceadaptersandindexingprimerstogeneratesequencing-readyDNAlibraries.PreparedlibrariesarethenloadeddirectlyontoaMiniSeq,MiSeqorNextSeqSystemforsequencing.
AnalysisSoftwareHighlights
TheAssign2.0TruSightHLAanalysissoftwareassistswiththeassignmentofahumanleukocyteantigen(HLA)type.ThesoftwareisdesignedtoanalyzedatafromlibrariespreparedwithIlluminaTruSightHLASequencingPanelsandthensequencedonanIlluminasequencer.UseAssign2.0toimportsequencedata,performbasecalling,editsequences,andcompareasampleconsensussequencewiththeIMGT/HLAdatabaseofalleles.
Specifications:
| AssayTime | 48hours |
| Hands-OnTime | 4.5hours |
| InputQuantity | 400ngDNA |
| SystemCompatibility | MiSeq,NextSeq550,MiSeqDxinResearchMode,MiniSeq,NextSeq500 |
| VariantClass | SingleNucleotidePolymorphisms(SNPs) |
| Technology | Sequencing |
| SpeciesCategory | Human |
| Method | HLASequencing |


