Cell Information:

Human Epidermal Keratinocytes (HEK) are primary cells isolated from normal human neonatal foreskin or adult skin. HEK is a suitable in vitro model for dermal toxicology tests in screening new skin products, and for developing dermal skin substitutes. HEK has been used in the following applications:

• Epidermal model generation
• Skin cancer
• Toxicity (skin absorption studies)
• Differentiation models (Ca++ free medium needed)
• Epidermal disease research
• Dermatitis/eczema, psoriasis, sebaceous cysts, folliculitis, and impetigo
• Dermal burns
• Wound healing

The Transfection Reagent:

Transfection of primary cells, such as HEK, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HEK. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for HEK. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent for transfection on HEK. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HEK
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

EZ Biosystems™是转染和基因表达产品及服务的全球供应商。我们的工厂位于马里兰大学帕克分校校园附近。公司致力于开发细胞类型特异性转染试剂，创造了世界上最大的细胞类型特异性转染试剂，可以在最常用的细胞系和原代细胞上进行最有效的转染。   产品 货号 规格 价格 Human Astrocyte (HA, NHA) Avalanche™  Transfection Reagent  EZT-HA00-1 0.5 ml $455.00 Human Astrocyte (HA, NHA) Avalanche™ Transfection Reagent  EZT-HA00-1 1.5 ml $795.00 Beta-TC-6 Cell Avalanche™ Transfection  Reagent (mouse pancreatic cancer cell) EZT-BETA-1 0.5 ml $455.00 Beta-TC-6 Cell Avalanche™ Transfection  Reagent (mouse pancreatic cancer cell) EZT-BETA-1 1.5 ml $795.00 H9c2(2-1) Cell Avalanche™ Transfection Reagent EZT-H9C2-1 0.5 ml $455.00 H9c2(2-1) Cell Avalanche™ Transfection Reagent EZT-H9C2-1 1.5 ml $795.00 Cementoblast (OCCM-30 cell) Avalanche™ Transfection Reagent EZT-OCCM-1 0.5 ml $455.00 Cementoblast (OCCM-30 cell) Avalanche™ Transfection Reagent EZT-OCCM-1 1.5 ml $795.00 Hep 3B Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) EZT-HEP3-1 0.5 ml $455.00 Hep 3B Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) EZT-HEP3-1 1.5 ml $795.00 Hep G2 Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell)  EZT-HEPG-1 0.5 ml $455.00 Hep G2 Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell)  EZT-HEPG-1 1.5 ml $795.00 Hepa 1-6 Cell Avalanche™ Transfection Reagent (mouse hepatoma cell)  EZT-HEPA-1 0.5 ml $455.00 Hepa 1-6 Cell Avalanche™ Transfection Reagent (mouse hepatoma cell)  EZT-HEPA-1 1.5 ml $795.00 HepG2/C3A Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) EZT-C3A0-1 0.5 ml $455.00 苏州蚂蚁淘代理EZ Biosystems™在中国的业务。试剂EZ Biosystems™代理北京，试剂EZ Biosystems™代理，EZ Biosystems™华东代理，EZ Biosystems™华北代理，EZ Biosystems™华中代理，EZ Biosystems™华南代理