Everest Biotech/Recombinant E. coli RNA Pyrophosphohydrolase/rppH 50μg/3712990501/50μg

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¥3900.00
货号:3712990501
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品牌:Everest Biotech
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商品描述

MessengerRNA(mRNA)degradationplaysakeyroleinthecontrolofgeneexpressioninallorganismsbylimitingthenumberoftimesthateachmRNAmoleculecanbeusedasatemplateforproteinsynthesis.RNApyrophosphohydrolase,alsocalledRppH,isamasterregulatorof5"-dependentmRNAdecay.Itacceleratesthedegradationoftranscriptsbyremovingpyrophosphatefromthe5"-endoftriphosphorylatedRNA,leADIngtoamorelABIlemonophosphorylatedstatethatcanstimulatesubsequentribonucleasecleavage.RppHpreferentiallyhydrolyzesdiadenosinepenta-phosphatewithATPasoneofthereactionproducts,andcanbeabletohydrolyzediadenosinehexa-andtetra-phosphate.However,thisproteinhasnoactivityondiadenosinetri-phosphate,ADP-ribose,NADHandUDP-glucose.InthemeningitiscausingstrainE.coliK1,ithasbeenshowntoplayaroleinHBMEC(humanbrainmicrovascularendothelialcells)invasioninvitro.

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Source

E.coli

Description

RecombinantE.coliRNApyrophosphohydrolaseisproducedbyourE.coliexpressionsystemandthetargetgeneencodingMet1-Gly176isexpressed.

Names

RNApyrophosphohydrolase,(Di)nucleosidepolyphosphatehydrolase,Ap5Apyrophosphatase,rppH

Accession#

P0A776

Formulation

Suppliedasa0.2μmfilteredsolutionof50mMTris,500mMNaCl,10%glycerol,pH8.0.

Shipping

Theproductisshippedondryice/icepacks.

Storage

Storeat<-20°C,stablefor6monthsafterreceipt.
Pleaseminimizefreeze-thawcycles.

Purity

Greaterthan95%asdeterminedbyreducingSDS-PAGE.

Endotoxin

Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest.

AminoAcidSequence
MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGESAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLLQLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQENTPKPQNASAYRRKRG