Clone | BL-2 |
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Size | 100μg/vial |
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Form | lyophilized |
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Igtype | mouseIgG1 |
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Specificity | Nocrossreactivitywithotherproteins. |
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Species | Human |
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Immunogen | Syntheticpeptidecorrespondingtoresidues41-54oftheBcl-2protein,conjugated |
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tothyroglobulin. |
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Contents | Mouseascitesfluid,1.2%sodiumacetate,2mgBSA,with0.01mgNaN3as |
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preservative. |
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Westernblot | At1-2μg/mlwiththeappropriatesystemtodetectBCL-2incellsandtissues. |
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Immunohistochemistry | At0.4-1μg/mltodetectBCL-2informalinfixedandparaffinembeddedtissues. |
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(Paraffin-embeddedSection) |
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Immunohistochemistry | At0.4-1μg/mltodetectBCL-2informalin/acetonefixedtissues. |
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(FrozenSection) |
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Immunocytochemistry | Suitable |
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Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.
Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.
1.Ngan,B.-Y.;Chen-Levy,Z.;Weiss,L.M.;Warnke,R.A.;Cleary,M.L.:Expressioninnon-Hodgkin"slymphomaoftheBCL-2proteinassociatedwiththet(14;18)chromosomaltranslocation.NewEng.J.Med.318:1638-1644,1988.
2.Hockenbery,D.;Nunez,G.;Milliman,C.;Schreiber,R.D.;Korsmeyer,S.J.:Bcl-2isaninnermitochondrialmembraneproteinthatblocksprogrammedcelldeath.Nature348:334-336,1990.
