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蚂蚁淘在线 / 品牌中心 / Everest Biotech / Everest Biotech/L-乳酸分析试剂盒I（10ml）-200次分析/1200012002/（10ml）-200次分析

Everest Biotech/L-乳酸分析试剂盒I（10ml）-200次分析/1200012002/（10ml）-200次分析

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￥1784.64

货号：1200012002

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商品描述

SampleTyple	Plasma,Serum,Cellculturesupernatant,otherbodyfluid
Contents
Method	Colorimetricmethodat490nm
StandardCurve
Sensitivity	60µM-3000µM
ReactionTime	30minutes
Applications	ForBIOLOGicalresearch: L-Lactatemeasurementinbiologicalsamples Fordrug/pharmresearch: DruginfluenceonL-Lactatemetabolism
Storage	-80°C
Notes	Materialsneededbutnotsupplied Aplatereadercapableofmeasuringabsorbancebetween470-490nm AdjustablePipettesandarepeatpipettor Asourceofpurewater;glassdistilledwaterofHPLC-gradewaterissufficient 0.5MAceticAcid Clearflatbottom96-wellplatesifnotincludedinthekitpurchased
Shipping	Icepacks

ReagentPreparation

Note:Allreagentsarefrozen.SWerecommendyouspinsmallvialsbeforeopening.

1.L-LactateStandards
Thevialcontains1000µlof3mML-LactateStandard.Thestandardmustbeequilibratedtoroomtemperaturebeforeuse.1mlofthestandardisenoughformaking3standardcurvesifassayedinduplicate.Storeat-80?C.

2.L-LactateAssaySolution
Thesolutioncontainsenzymesthatarelightsensitive.Thesolutionmustbethawedonicebeforeuse.Besttoaliquottheamountneededanduseitalltopreventthawing/freezingcycles.Freezeandstorealiquotsat-80oC

Protocol

1.SamplePreparation

Serum/Plasma/otherbodyfluid/cellculturesupernatant
Serum,Plasma,otherbodyfluid,orcellculturesupernatantcanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;.…..)toensurethereADIngsarewithinthestandardcurve.
YoursamplescanbedilutedwithdH2O.
Note:ifsamples(likehemolyzedserum/plasmaorcellculturemediumwhichcontainFBS)containhighleveloflactatedehydrogenasecapableofconvertinglactatetopyruvate,itisimportantforthesamplestobedeproteinated.

SolidSamples
Solidsamples,suchastissues,canbefirsthomogenizedandextractedwithethanol(80%)withatissues/Ethanolratioof1:8(1hrat4°C)followedbycentrifugationat10,000xg.Theclearsupernatantsthencanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;.…..)toensurethereadingsarewithinthestandardcurve.YoursamplescanbedilutedwithdH2O.

AddingSamples
Add50μlofsamplestoeachwell.
Werecommendthatsamplesbeassayedinduplicate.

Note:
Ifpreparedsamplesarenotassayedthesameday,storethesamplesat-80?C.Ifsamplesneedtobedeproteinated,makesuretodeproteinizethesamplespriortostoringinthefreezer.Thedeproteinatedsampleswillbestableforonemonthwhilestoredat-80?C.Forfrozensamples,dilutionsofsamplesmustbedonerightbeforeassaying.

2.StandardCurvePreparation
WerecommendthatL-LactateStandardsbeassayedinduplicate.Astandardcurvehastoberunineachassay.
Add50µl,40µl,30µl,20µl,10µl,5µl,1µl,and0µlofL-lactateStandardtoeachwell.Thenadjustvolumeto50μl/wellwithdH2O.

3.Performtheassay

a)Add50ulofL-LactateassaysolutiontoeachwellcontainingL-Lactatestandardsandtestsamples.
b)Incubatefor30minsat37°Cincubator.Note:PleasedonotuseCO2incubator.
c)Stopthereactionbyadding50μlof0.5Maceticacidperwellfollowedbybriefgentleagitation.Note:Eliminateanyairbubblespresentinthewellsusinganeedlepriortomeasurement.
d)Measuretheabsorbanceat490nMusingamicroplatereader.

4.Calculations

a)AveragetheOD490nmvaluesofreplicatewellsofeachL-Lactatestandard,testsamples,andblank.Inordertogetthecorrectedabsorbance,subtracttheaverageOD490nmvalueoftheblank(L-LactateStandard#8)fromtheaverageOD490nmvaluesfromallstandardsandsamples.

b)MakeastandardcurvebyplottingOD490nmvaluesfromeachL-LactatestandardsasafunctionofL-Lactateconcentration.Thiscanbedonewithexcelspreadsheet.CalculatethevalueofL-Lactateinsamplesusingtheequationobtainedfromthelinearregressionofthestandardcurve.

L-Lactate(µM)=[(Correctedabsorbance)-(y-intercept)]/slope

MaterialSafetyDataSheet

Date:Oct26,2015

1.PRODUCTANDCOMPANYIDENTIFICATION

1.1Productidentifier

Productname:L-LactateAssayKitI

CatalogNo:1200011002,120001100A,120001100P,1200012002,120001200A,12001200P,1200014002,120001400A,120001400P

1.2Relevantidentifiedusesofthesubstanceormixtureandusesadvisedagainst

Forresearchuseonly.

1.3Detailsofthesupplierofthesafetydatasheet

Company:EtonBioscienceInc

TollFree:1-800-758-1630

Tel:1-800-758-1630

Fax:1-800-507-2912

1.4Emergencytelephonenumber

1-800-758-1630

2.HAZARDSIDENTIFICATION

2.1Classificationofthesubstanceormixture

Notahazardoussubstanceormixture

2.2GHSLabelelements,includingprecautionarystatements

Notahazardoussubstanceormixture

2.3Otherhazards

None

3.COMPOSITION/INFORMATIONONINGREDIENTS

3.1Substances

4.FIRSTAIDMEASURES

4.1Descriptionoffirstaidmeasures

Eyecontact

Checkforandremovecontactlensesandflushwithcopiousamountsofwater;assureadequateflushingbyseparatingtheeyelidswithfingers;callaphysician

SkinContact

Flushwithcopiousamountsofwater;removecontaminatedclothingandshoes;callaphysician

Inhalation

Removetofreshair.Ifnotbreathinggiveartificialrespiration;callaphysician

Ingestion

Ifswallowed,nevergiveanythingbymouthtoanunconsciousperson.Rinsemouthwithwater;callaphysician

4.2Mostimportantsymptomsandeffects,bothacuteanddelayed

Noinformationavailable

4.3Indicationofanyimmediatemedicalattentionandspecialtreatmentneeded.

Noinformationavailable

5.FIREFIGHTINGMEASURES

5.1Extinguishingmedia

Suitableextinguishingmedia

Waterspray,alcohol-resistantfoam,drychemicalorcarbondioxide

5.2Specialhazardsarisingfromthesubstanceormixture

Noinformationavailable

5.3Adviceforfirefighters

Wearself-containedbreathingapparatusandprotectiveclothingtopreventcontactwithskinandeyes.

6.ACCIDENTALRELEASEMEASURES

6.1Personalprecautions,protectiveequipmentandemergencyprocedures

Usefullpersonalprotectiveequipment.Evacuatepersonneltosafeareas.Avoidbreathingvapors,mistorgas.

6.2Environmentalprecautions

Preventfurtherleakageorspillage.Preventproductfromenteringdrain.

6.3Methodsandmaterialforcontainmentandcleaningup

Keepinsuitable,closedcontainersfordisposal

7.HANDLINGANDSTORAGE

7.1Precautionsforsafehandling

Avoidskin/eyecontact.Useprotectiveequipmentasneeded.Washcontaminatedclothingbeforereuse.

7.2Conditionsforsafestorage,includinganyincompatibilities

Keepcontainertightlyclosed.Storeat-80?C.

7.3Specificenduse(s)

Nodataavailable.

8.EXPOSURECONTROLS/PERSONALINFORMATION

8.1ControlParameters

Thisproductcontainsnohazardousmaterialswithoccupationalexposurelimits.

8.2Exposurecontrols

Engineeringcontrols

Provideshowerandeyewashstation

Personalprotectiveequipment

	L-lactateAssaySolution
	Components		CAS#
	DMSO		67-68-5
	L-LDH		9001-60-9
	Tris-HCl,pH7.5		N/A
	Water		7732-18-5
	L-LactateAssayStandard
	Components		CAS#
	SodiumL-Lactate		867-56-1
	Tris-HCl,pH7.5		N/A
	Water		7732-18-5

EyeProtection	Wearsafetygoggles
SkinProtection	Wearprotectiveclothing
HandProtection	Wearprotectivegloves
Respiratoryprotection	WearNIOSH/MSHAapprovedrespirators

9.PHYSICALANDCHEMICALPROPERTIES

9.1Informationonbasicphysicalandchemicalproperties

L-LactateAssaySolution

Appearance	Liquid,yellowish
pH	7.5
Meltingpoint	Nodataavailable
Freezingpoint	Nodataavailable
Boilingpoint	Nodataavailable
Density	Nodataavailable
Watersolubility	Soluble

L-LactateStandard

Appearance	Liquid,colorless
pH	7.5
Meltingpoint	Nodataavailable
Freezingpoint	Nodataavailable
Boilingpoint	Nodataavailable
Density	Nodataavailable
Watersolubility	Soluble

9.2Othersafetyinformation

Nodataavailable.

10.STABILITYANDREACTIVITY

10.1Reactivity

Nodataavailable

10.2Chemicalstability

Stableunderrecommendedstorageconditions.

10.3Possibilityofhazardousreactions

Nodataavailable.

10.4Conditionstoavoid

Nodataavailable

10.5IncompatIBLematerials

Strongoxidizingagents

10.6Hazardousdecompositionproducts

Hazardousdecompositionproductsformedunderfireconditions:carbonoxides

11.TOXICOLOGICALINFORMATION

11.1Informationontoxicologicaleffects

Toxicologicaleffectsonthisproducthavenotbeenthoroughlystudied.

12.ECOLOGICALINFORMATION

12.1Toxicity

Avoidreleaseintoenvironment

12.2Persistenceanddegradability

Nodataavailable

12.3.Bioaccumulativepotential

Nodataavailable

12.4Mobilityinsoil

Nodataavailable

12.5ResultsofPBTandvPvBassessment

Nodataavailable

12.6Otheradverseeffects

Nodataavailable

13.DISPOSALINFORMATION

13.1Wastetreatmentmethods

Disposeinaccordancewithlocal,state,andfederalregulations.

14.TRANSPORTINFORMATION

DOT:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
IATA:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

ADR/RID

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

15.REGULATORYINFORMATION

EURiskandSafetyphrases:

R36/37/38:irritatingtoeyes/respiratorysystem/skin

16.OTHERINFORMATION

Theinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.

Q.WhattypeofmediumshouldIuseformakingculturedcellsforLactateassay?

A.Pleaseusephenolredfreemedium.Pleasedonotusephenolredmediumsincephenolredwouldaffectabsorbancereadings.

Q.WhatenzymesisusedinLactateAssay?

A.ItisLDH.

Q.CanyoutellmethecompositionsofyourLactateAssay?

A.Unfortunatelyitisproprietary.

Q.HowdoIknowwhetherIneedtouseL-LactateorD-Lactateassaykit?

A.L-Lactateassaykitsarecommonlyusedformeasuringlactatelevelinanimalcells.

D-lactateassaykitsarecommonlyusedformeasuringlactatelevelinbacteriacells.

Q.DoIneedtomakeastandardcurveeverytime?

A.Yes,itisnecessarysinceyoucalculateyoursampleconcentrationbasedonthestandardcurve.PleasedonotusetherepresentativeLactatestandardcurveintheprotocoltocalculateconcentrationsofyoursamples.

Q.CanIusefluorescencespectroscopytomeasurereadingsforLactateassaykits?

A.No,thekitdoesnotworkwithafluorescencereadersincethekitemployscolorimetricassays.

Q.HowdoImeasureLactatefromculturedcells?

A.Youcangrowcellsinphenolred-freemedium,thenyoucanextractcellswith80%ethanol.

Q.CanIusefrozensamples?

A.Althoughitisbettertousefreshsamplesforassays,youcanusefrozensamples.Ifyouarenotusingfrozensamplesinonce,pleasemakealiquotsofyoursamplesbeforeyouputtheminafreezertopreventfromdegradingsamplesfromrepeatedfreeze-thawcycles.

Q.Mysampleformedpreciptationafteraddingaceticacid.HowcanIpreventthis?

A.Youcanstillmeasurereadingswithoutaddingaceticacid.

Q.Theprotocolrecommendsmeasuringtheabsorbanceat490nm.Is485nmcloseenough?

A.Itisokaslongasyousetuptheabsorbancebetween470and490nm.

Q.WillL-LactateassaymeasureD-Lactate?

A.No,itwont.

Q.CanIuseatransparentwall96-wellplatewithLactateKit?

A.Yes,youcan.

Q.CanEthanolinterferewiththeenzyme?

A.Theenzymecantolerateupto20%ofethanolwithoutaffectingit’senzymaticactivitywithL-Lactate.

SamVandeVelde,MeghanF.Hogan,andMarcMontminy.:mTORlinksincretinsignalingtoHIFinductioninpancreaticbetacells.PNAS,Oct2011;108:16876-16882.

JunMifune,KatrinGrage,andBerndH.A.Rehm.:ProductionofFunctionalizedBiopolyesterGranulesbyRecombinantLactococcuslactis.Appl.Envir.Microbiol.,Jul2009;75:4668-4675.

DragutinJ.Savic,WilliamM.McShan,andWilliamM.McShan.:Long-termsurvivalofStreptococcuspyogenesinrichmediaispH-dependent.Microbiology,Jun2012;158:1428-1436

KristinA.Anderson,FuminLin,ThomasJ.Ribar,RobertD.Stevens,MichaelJ.Muehlbauer,ChristopherB.Newgard,andAnthonyR.Means.:DeletionofCaMKK2fromtheLiverLowersBloodGlucoseandImprovesWhole-BodyGlucoseToleranceintheMouse.Mol.Endocrinol.,Feb2012;26:281-291.

JaninaP.Lewis,DivyaIyer,andCeciliaAnaya-Bergman.:AdaptationofPorphyromonasgingivalistomicroaerophilicconditionsinvolvesincreasedconsumptionofformateandreducedutilizationoflactate.Microbiology,Nov2009;155:3758-3774.

AmparoWolf,SameerAgnihotri,JohannMicallef,JoydeepMukherjee,NesrinSabha,RobCairns,CynthiaHawkins,andAbhijitGuha.:Hexokinase2isakeymediatorofaerobicglycolysisandpromotestumorgrowthinhumanglioblastomamultiforme.J.Exp.Med.,Feb2011;208:313-326.

InnaSerganova,AsifRizwan,XiaohuiNi,SunithaB.Thakur,JelenaVider,JamesRussell,RonaldBlasberg,andJasonA.Koutcher.:MetabolicImaging:ALinkbetweenLactateDehydrogenaseA,Lactate,andTumorPhenotype.Clin.CancerRes.,Oct2011;17:6250-6261.

ChangluLiu,JiejunWu,JessicaZhu,ChesterKuei,JingxueYu,JonathanShelton,StevenW.Sutton,XiaorongLi,SuJinYun,TaranehMirzadegan,CurtMazur,FredrikKamme,andTimothyW.Lovenberg.:METABOLISM,REGULATION,ANDSIGNALING:LactateInhibitsLipolysisinFatCellsthroughActivationofanOrphanG-protein-coupledReceptor,GPR81.J.Biol.Chem.,Jan2009;284:2811-2822.

MarinaOstroukhova,NicholasGoplen,MdZunayetKarim,LidiaMichalec,LeiGuo,QiaolingLiang,andRafeulAlam.:Theroleoflow-levellactateproductioninairwayinflammationinasthma.AmJPhysiolLungCellMolPhysiol,Feb2012;302:L300-L307.

Zhuang,Yongxian,DanielKChan,andWKeithMiskimins.“PreventingFeedbackActivationofGlycolyticATPProductionEnhancesMetforminCytotoxicityinBreastCancerCellsWhenOxidativePhosphorylationIsInhibited.”Cancer&Metabolism2.Suppl1(2014):P89.PMC.Web.6Oct.2015.

Lezi,E.etal.“EffectofExerciseonMouseLiverandBrainBioenergeticInfrastructures.”Experimentalphysiology98.1(2013):207–219.PMC.Web.6Oct.2015.

YongxianZhuang,DanielK.Chan,AllisonB.Haugrud,W.KeithMiskimins.MechanismsbyWhichLowGlucoseEnhancestheCytotoxicityofMetformintoCancerCellsBothInVitroandInVivo.September25,2014DOI:10.1371/journal.pone.0108444

BorgesFT1,MeloSA,ÖzdemirBC,KatoN,RevueltaI,MillerCA,GattoneVH2nd,LeBleuVS,KalluriR.TGF-β1-containingexosomesfrominjuredepithelialcellsactivatefibroblaststoinitiatetissueregenerativeresponsesandfibrosis.JAmSocNephrol.2013Feb;24(3):385-92.doi:10.1681/ASN.2012101031.Epub2012Dec28.

Everest Biotech的山羊多克隆抗体以Everest Elite Grade或Aspiring Grade产品的形式发布，这取决于它们的测试性能和对靶蛋白的了解程度。Everest Biotech提供了圣克鲁斯多克隆产品系列的高质量替代品精英级抗体保证按数据表上的规定工作，或退款。肽ELISA数据显示它们可以识别免疫肽。Western印迹数据显示生理相关组织或细胞系上的正确大小带。IHC / IF数据和特定出版物（如果有）。100％满意保证也可批量提供给化验开发人员有抱负的抗体快速将市场上几乎没有或没有商业抗体可用且在文献中几乎没有表达数据的靶标引入市场。由肽ELISA数据支持。背景低但带大小错误或在可用于测试的常见细胞系和组织范围内没有信号。以大幅降低的价格出售，但我们不确定他们会在任何应用中识别天然蛋白质。100％满意保证奖励分享显示抗体成功发挥作用的数据-将您的数据提交到support@everestbiotech.com可获得您选择的免费抗体。免疫肽产生抗体的肽可用于封闭实验以100µg冻干肽的形式出售

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