Sorafenibisanovelbi-arylureacompoundthatinhibitscellproliferationbytargetingtheERKpathwayandangiogenesisbytargetingthereceptortyrosinekinasesVEGFR-2andPDGFR-ßandtheirassociatedsignalingcascades.AlthoughsorafenibwasinitiallydevelopedasaRafkinaseinhibitor(IC50=6nM),ithassincebeenshowntohaveactivityagainstmanyreceptortyrosinekinasesinvolvedintumOrigenesisandangiogenesisincludingFGFR-1,wtBRAFandV599EmutantBRAF,aswellasmembersoftheso-called"splitkinase"family:VEGFR-2,VEGFR-3,PDGFR-ß,c-KIT,andFlt3.However,sorafenibisnotactiveagainsterbB1,erbB2,ERK-1,MEK-1,EGFR,HER-2,IGFR-1,c-MET,c-yes,PKB,PKA,cdk1/cyclinB,PKC,andpim-1.IncellularmechaNISTicassays,sorafenibdecreasedbasalphosphorylationoftheERKpathwayinmelanoma,breast,colon,andpancreatictumorcelllines. Eton Bioscience我们的DNA制备服务可以从在琼脂平板上培养的质粒DNA或菌落制备DNA。如有必要，我们将执行转换步骤。完成后，我们将提供最终产品以及服务摘要，其中包括最终DNA浓度，体积和分光光度DNA分析