Transfection - EZ Biosystems: Cell Lines

Cell LineInformation:

Designations: SK-N-SH Organism: Homo sapiens (human) Tissue: neuroblastoma; brain; derived from metastatic site: bone marrow Age: 4 years Gender: female Morphology: epithelial Growth properties: adherent AntigenExp: Blood Type A; RhProducts: plasminogen activator shows increased expression of M-CSF after treatment with amyloid-beta peptide Depositors: G. Trempe; L.J. Old The SK-N-SH line was developed by J.L. Biedler. SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays.

The Transfection Reagent:

SK-N-SH Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SK-N-SH Cells. The proprietary formulationof lipids and polymersensures the highest possible transfection efficiencies and viabilities for SK-N-SH Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into SK-N-SH Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SK-N-SH cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SK-N-SH cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SK-N-SH Cell Avalanche Transfection Reagent.

For Other Cells:

SK-N-SH Cell Avalanche™ Transfection Reagent (human neuroblastoma cell) can also be used on the following cells with high transfection efficiencies. LA-N-2 IMR-32 786-O Cell Caki-1 Cell MDCK Cell Vero Cell DU 145 Cell PC-3 Cell AR42J Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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