雅典研究院/坎比奥-卓越分子生物学/50µ摩尔/13-1233-95

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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

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GTT Trimer Phosphoramidite

GTT Trimer Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
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13-1233-90GTT Trimer Phosphoramidite100µmoles£680.00£646.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
13-1233-95GTT Trimer Phosphoramidite50µmoles£340.00£323.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

GTT Trimer Phosphoramidite

GTT Trimer Phosphoramidite

Glen Research

GTT Trimer Phosphoramidite

Structure

Catalog Number: 13-1233-xx

Description: GTT Trimer Phosphoramidite

5"-Dimethoxytrityl-N2-isobutyryl-2"-deoxyGuanosine-3"->5"-o-chlorophenyl-phosphoryl-2"-deoxyThymidine-3"->5"-o-chlorophenyl-phosphoryl-2"-deoxyThymidine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C76H86Cl2N11O22P3M.W.: 1667.5

Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Coupling:15 minute coupling time recommended.
Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

EXAMPLE OF PREPARATION OF TRIMER MIXTURE

Prepare 530 mg of the trimer mix, taking the amount (mg) for each trimer from the right column. Dissolve the trimer mix in dichloromethane (highest grade possible; acid-free). Evaporate to dryness to produce a homogenous mixture of all 20 trimers.

EXAMPLE OF PREPARATION OF TRIMER MIXTURE FOR THE SYNTHESIZER

Dissolve 530 mg, which is equivalent to 20X10 µmoles (normalized for RF) of the trimer mix in 2.0 mL of acetonitrile-dichloromethane mixture, 1:3 v/v to produce a 0.10N solution of trimers, ready for use in a synthesizer.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

GTT Trimer Phosphoramidite

GTT Trimer Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 16.2: TRIMER (CODON) PHOSPHORAMIDITES SIMPLIFY LIBRARY PREPARATION
Glen Report 18.1: Trimer Phosphoramidites - Tools for fine-tuning protein function

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

GTT Trimer Phosphoramidite

GTT Trimer Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.

ABI 392/394
Cat.No.PackSizeGrams/Pack0.1M Dil.(mL)LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
13-1233-90100µmoles.167grams120127.55.4541
13-1233-9550µmoles.083grams.53.3321.25.91.67.17
Expedite
Cat.No.PackSizeGrams/PackDilution(mL)Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
13-1233-90100µmoles.167grams1.5.0723.614.7510.731.48
13-1233-9550µmoles.083grams.75.078.65.383.91.54
Beckman
Cat.No.PackSizeGrams/PackDilution(mL)Molarity30nm200nm1000nm
Approximate Number of Additions
13-1233-90100µmoles.167grams1.5.0725.215.7511.45
13-1233-9550µmoles.083grams.75.0710.26.384.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

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