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蚂蚁淘在线 / 品牌中心 / Alomone / 阿洛蒙/抗AKAP150抗体/APZ-031/25µl

阿洛蒙/抗AKAP150抗体/APZ-031/25µl

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￥4940.00

货号：APZ-031-25µl

浏览量：127

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商品描述

Cat#:APZ-031

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:m,r

Immunogen

Peptide(C)RGAKRSRPSKLTED,correspondingtoaminoacidresidues128-141ofratAKAP150(Accession P24587).Intracellular.

Accession(Uniprot)NumberP24587

GeneIDP24587

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyMouse–13/14aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

SpecificityTheantibodywon’trecognizethehumanAKAP150homologue(alsoknownasAKAP79).

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.8mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20ºC.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:wb

Mayalsoworkin:ic,ifc,ih,ip

Westernblot

Westernblotanalysisofratbrainmembranes(lanes1and5),mousebrainmembranes(lanes2and6),ratdorsalrootganglionlysate(lanes3and7)andmouselunglysates(lanes4and8):

1-4. Anti-AKAP150Antibody(#APZ-031),(1:200).
5-8.Anti-AKAP150Antibody,preincubatedwiththecontrolpeptideantigen.

References

Schwartz,J.H.(2001)Proc.Natl.Acad.Sci.USA.98,13482.
Feliciello,A.etal.(2001)J.Mol.Biol.308,99.
Adler,E.M.(2014)J.Gen.Physiol.143,313.
Esseltine,J.L.andScott,J.D.(2013)Trends.Pharmacol.Sci.34,648.
Ting,J.T.etal.(2012)Annu.Rev.Neurosci.35,49.
Nieves-Cintron,M.etal.(2015)Cell.Signal.Onlinepublication.

Scientificbackground

A-Kinaseanchorproteins(AKAPs)areagroupofstructurallydiverseproteinsthatbindtotheregulatoryunitofProteinKinaseA(PKA)todiscretelocationswithinthecell¹.AKAPsincludetwoconservedstructuralmodules:AtargetingdomainthatservesasascaffoldandmembraneanchorthatconsistsoftwoNH2-terminalstructuralmodulesthatarehighlyconservedamongmammalianhomologues.TheyalsohaveatetheringdomainthatinteractswithPKAregulatorysubunitswhichconsistsofashortamphipathichelix.AlternativesplicingcanshuffletargetingandtetheringdomainstogenerateavarietyofAKAPswithdifferenttargetingspecificity.AlthoughAKAPshavebeenidentifiedonthebasisoftheirinteractionwithPKA,theyalsobindothersignalingmolecules,mainlyphosphatasesandkinasesthatregulateAKAPtargetingandactivateothersignaltransductionpathways².

SometypesofAKAPsinteractwith Ca_V1.1 and Ca_V1.2.ThesevoltagegatedcalciumchannelsarephosphorylatedbyPKAafterβ-adrenergicactivation.ThisphosphorylationisenabledbythebindingofAKAP150tothedistalC-terminalofCa_V1.1andCa_V1.2.BindingofdifferentAKAPsthatinteractwithdistinctsignalingpartnerstothecalciumchannelintracellularC-terminaldomainmayacttofine-tunechannelactivityanddownstreamcalcium-mediatedprocesses³.

AsinglesubtypeofneuronalAKAPisexpressedwidelyinthebrainandishighlyconservedinmouse(150kDA),bovine(75kDA)andhuman(79kDA).APAK150targetsPKAIItothepostsynapticCytoskeletonandperikaryaofneurons.ItisexpressedinPurkinjecellsandinolfactorybulbneurons,basalganglia,cerebralcortexandotherforebrainareas.AKAP150isconcentratedinprimarybranchesofdendritesinassociationwithmicrotubules.ThelocalizationofPKAIIβinbrainoverlapswiththatofAKAP150,suggestingthatAKAP150istherelevantPKAIIβanchorproteininthebrain².

InhibitionofeitherAKAP150or TRPV1 bindingsitescausesanabolishmentofPKC-dependentTRPV1sensitizationtocapsaicinstimulithusmakingtheAKAP150-TRPV1interactionaviabletargetfornovelanalgesics⁴.

AKAP150interactswithanothergroupofscaffoldingproteinsknownastheSAP90/PSD95-associatedproteinfamily.AKAP150interactsdirectlywiththeSH3andGKdomainsof PSD95 and SAP97.Theseproteinshavespecificrolesinsynapticlocalizationof NMDARs and AMPARs respectively⁵.

Postmyocardialinfarction,AKAP150-mediatedtargetingofcalcineurintosarcolemmalmicro-domainsinventricularmyocytescontributestothelocalandacutegeneremodelingeventsthatleadtothedown-regulationof K_V currents⁶.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:06/03/2019

Anti-AKAP150Antibody(#APZ-031)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblotanalysis.IthasbeendesignedtorecognizeAKAP150frommouseandratsamples.TheantibodywillunlikelyrecognizeAKAP150fromhumansamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofratbrainmembranes(lanes1and5),mousebrainmembranes(lanes2and6),ratdorsalrootganglionlysate(lanes3and7)andmouselunglysates(lanes4and8):

1-4. Anti-AKAP150Antibody(#APZ-031),(1:200).
5-8.Anti-AKAP150Antibody,preincubatedwiththecontrolpeptideantigen.

Alomone Labs是您离子通道研究的合作伙伴，是离子通道，GPCR和神经信号工具的领先供应商。我们专门生产基础科学研究中使用的一抗，小分子，蛋白质和肽。Alomone成立于1989年，现已成为全世界科学家使用的最全面的离子通道研究工具来源。Alomone的所有产品均经过生物测定验证，并以冻干粉形式提供，因此您可以对它们的生物活性和长保质期充满信心。立即索取信息 Alomone公司作为世界上***的离子通道专业供应商，它的离子通道抗体是世界上*全的、质量也非常好！目前被世界各地的科学家广泛的使用. Alomone Labs Alomone 公司是一家位于耶路撒冷的以色列著名的生物科技公司，专著于提供生命科学细胞生物学试剂和服务。Alomone 公司作为世界上最著名的离子通道专业供应商，它的离子通道抗体是世界上最全的、质量也非常好！目前被世界各地的科学家广泛的使用。试剂

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阿洛蒙/抗AKAP150抗体/APZ-031/25µl

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