快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Accelagen / 促进剂/涡轮生物素化TM试剂盒/50X涡轮生物素化酶（0.05 mg）
10X涡轮生物素化混合物（1 ml）/B0114P

促进剂/涡轮生物素化TM试剂盒/50X涡轮生物素化酶（0.05 mg）
10X涡轮生物素化混合物（1 ml）/B0114P

价格
￥5960.00
货号：B0114P
浏览量：127
品牌：Accelagen
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Accelagen TurboBiotinylation Kit, the best biotinylation kit
The Gene-to-Protein Company
Request Quote
Protein PurificationBaculovirus ExpressionMammalian ExpressionE. coli ExpressionMolecular BiologyProducts

Products: Eznyme Toolbox: TurboBiotinylation Kit

TurboBiotinylationTM Kit

Catalog #:Size:Concentration: Price:
B0114P 50X TurboBiotinylase (0.05 mg) 10X TurboBiotinylation Mix (1 ml) 2.5 mg/ml $298
B0114M 50X TurboBiotinylase (0.5 mg) 10X TurboBiotinylation Mix (1 ml) 2.5 mg/ml $1,678
B0114L 50X TurboBiotinylase (2.5 mg) 10X TurboBiotinylation Mix (5 ml) 2.5 mg/ml $5,400
T0114B Bulk 2.5 mg/ml Inquiry
To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490
PRODUCT DETAILDATA SHEETPROTOCOL
Components and Formulation:
  1. 50X TurboBiotinylase at 2.5 mg/ml (80 µM) in Storage Buffer of 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM TCEP, 50% glycerol.
  2. 10X TurboBiotinylation Mix (50 mM Tris-HCl, pH 7.5, 100 mM MgCl2, 100 mM ATP, 5 mM biotin)
TurboBiotinylase Purity:
TurboBiotinylase is a stable ultra-pure recombinant E. coli BirA biotin ligase purified from E. coli and has a calculated molecular weight of 35.6 kDa. It is free of protease or nuclease contamination.
TurboBiotinylase Activity:
The efficiency of biotinylation can be measured by mass spectrometry. Biotinylation adds 226.31 Da to a protein.

Alternatively, biotinylation can be evaluated by streptavidin-agarose pull-down. A Coomassie stained gel will visually show the efficiency of biotinylation.

  1. Remove biotin and ATP from the biotinylation reaction sample by a spin desalting column.
  2. Save sample as Total (T)
  3. Incubate with streptavidin-agarose for 1 hour with agitation.
  4. Spin down the streptavidin-agarose.
  5. Save the supernatant as Unbound (U)
  6. Save the streptavidin-agarose as Bound (B)
  7. Run gel and stain with Coomassie Blue.

Protein Purification |Baculovirus Expression | Mammalian Expression |E. coli Expression |Molecular Biology | Proteins

tel: 858-678-8618fax: 888-239-6490 toll-free: 888-365-8085email: info@accelagen.comCopyright © 2020 Accelagen Inc.All rights reserved.

Accelagen Accelagen 是一个快速成长起来的gene-to-protein公司。可提供gene-to-protein全方位解决方案。该公司生产的重组蛋白已经广泛应用于基 于结构的药物设计(SBDD)和高通量筛选(HTS)领域，Turbo3C(HRV3C)Protease, TurboTEV Protease, TurboNuclease是该公司极具性价比的优质产品。Accelagen重组蛋白表达和纯化服务可为生物学科研人员及药物研究提供高品质的重组蛋 白。