BPS Bioscience蚂蚁淘在线

Description:

NF-ĸBluciferasereporterconstructisstablyintegratedintothegenomeofJurkatT-cells.Thefireflyluciferasegeneiscontrolledby4copiesofNF-kBresponseelementlocatedupstreamoftheTATApromoter.Followingactivationbystimulants,endogenousNF-ĸBtranscriptionfactorsbindtotheDNAresponseelementstoinducetranscriptionoftheluciferasegene.

MaterialsRequiredButNotProvided:

TNFα(Sigma,#T0157-10UG)
PMA(LCLaboratories,#P1680)
Ionomycin(Sigma,#I3909)
AssayMedium:ThawMedium2(BPSCat.#60184)
96-welltissueculturetreatedwhiteclear-bottomassayplate(Corning#3610)
ONE-Stepluciferaseassaysystem(BPSCat.#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity
Luminometer

Synonym(s):

nfkb

HostCellline:

HumanAcuteT-CellLeukemicCellLine(CloneE61).SUSPensioncells.

MycoplasmaTesting:

ThiscelllinehasbeenscreenedusingtheMycoAlert™MycoplasmaDetectionKit(Cat.No.LT07-118)toconfirmtheabsenceofMycoplasmacontamination.MycoAlertAssayControlSet(Cat.No.LT07-518)wasusedasapositivecontrol.

Format:

Eachvialcontains~2x10^6cellsin1mLof10%DMSOinFBS.

Storage/StABIlity:

Storeinliquidnitrogenimmediatelyuponreceipt.

Application(s):

TheNF-ĸB-luciferase/JurkatcelllineissuitableformonitoringtheactivityofNF-ĸBsignalinginresponsetostimulants,andestablishingcell-basedscreensforinhibitorsthattargetspecificNF-ĸB-stimulatingmolecules.

Reference(s):

1.BornerCandKrausJ(2013)InhibitionofNF-kBbyOpioidsinTcells.JImmunology.191.

2.MoriN.et.al.(2002)Bay11-7082inhibitstranscriptionfactorNF-kBandinducesapoptosisofHTLV-1-infectedTcelllinesandprimaryadultT-cellleukemiacells.Blood.100:1828-1834

3.TakadaY,KobayashiY,AggarawalBB(2005)EvodiamineabolishesconstitutiveandinducIBLeNF-kappaBactivationbyinhibitingIkappaBalphakinaseactivation,therebysuppressingNF-kappaB-regulatedantiapoptoticandmetastaticgeneexpression,up-regulatingapopotissandinhibitinginvasion.JBiolChem.280:17203

Notes:

Contactusforpricing.

LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.

ScientificCategory:

Immunotherapy

ProductType:

CellLine

Datashownislot-specific.Contactusforspecificinformationonotherlots.

BPS Bioscienc 50272说明书点击次数：744 发布时间：2018-08-06 美国BPS Bioscience公司是一家快速发展的企业，总部在California.产品远销世界各地.BPS提供产品和服务,主要方向是蛋白激酶,磷酸激酶，HDAC等.BPSzui大的药物发现用重组酶的供应商，并提供蛋白表达和基于细胞和生物化学检测的产品定制服务和化合物筛选服务。BPS在不断扩大其药物发现产品。BPS还提供了自定义的蛋白表达，生物化学和基于细胞的检测，化合物筛选服务。 BPS是顾客导向。以及为客户提供服务，我们倾听您的需要定制我们的服务，以满足您的具体情况。通过BPS的重点在技术发展的zui前沿，的质量生命科学产品和服务提供及时，这将有助于我们的客户加快药物发现和开发用于治疗人类疾病。 BPSBioscience BCL-2, His-tagCatalog #: 50272Size: 100 µg 物种：人类宿主物种：大肠杆菌MW：27 kDaGenbank＃：P10415标签：C端His标签aa：1-211背景：抑制多种细胞系统中的细胞凋亡，包括因子依赖性淋巴造血细胞和神经细胞。通过控制线粒体膜通透性来调节细胞死亡。似乎在具有半胱天冬酶的反馈回路系统中起作用。通过阻止细胞色素c从线粒体释放和/或通过结合凋亡激活因子（APAF-1）来抑制胱天蛋白酶活性。描述：人Bcl-2（GenBank登录号P10415），aa 1-211，具有C末端His标签，MW = 27kDa，在大肠杆菌表达系统中表达。UniProt的P10415同义字（词）：Bcl，Bcl2，B细胞淋巴瘤2检测条件：遵循BPS Cat的协议。＃50222。简言之，将BCL-2与TR-FRET测定缓冲液中的BCL-2肽配体以及抗His Tb供体和染料标记的受体组合。加入不同浓度的ABT 199，将反应物在室温下温育2小时，然后进行荧光强度测量。公式：40 mM Tris-HCl，pH 8.0,110 mM NaCl，2.2 mM KCl，3 mM DTT，20％甘油和200 mM咪唑格式：水性缓冲溶液应用程序（S）：可用于细胞凋亡，筛选抑制剂和选择性分析的研究。参照（一个或多个）：1. Labi V.等，Cell Death Differ。2008年6月; 15（6）：977-87。2. Rong YP等人，Mol Cell。2008年7月25日; 31（2）：255-65。警告（S）：避免冻/融循环科学类别：细胞凋亡产品类别：重组蛋白货号品名规格品牌79531Growth Medium 1B500mlbpsbioscience50272BCL-2, His-tag100 µgbpsbioscience50273BCL-XL, His-tag100 µgbpsbioscience100077BCL2A1, His-Tag100 μgbpsbioscience100080BCL2L10, His-Tag100 µgbpsbioscience100091BCL2L2, His-Tag100 µgbpsbioscience80500Caspase3, His-tag50 µgbpsbioscience80113Caspase6, His-tag50 µgbpsbioscience70000Caspase7, His-tag50 µgbpsbioscience80114Caspase8, His-tag50 µgbpsbioscience80115Caspase9, His-tag50 µgbpsbioscience40742MCL1, His-Tag100 µgbpsbioscience80117NLRP3 (NALP3), His-FLAG-tags5 µgbpsbioscience75003STAT3, GST-tag20 µgbpsbioscience80016WDR5, His-Tag, Biotin-Labeled50 μgbpsbioscience75002XIAP (Bir2,Bir3), His-tag50 µgbpsbioscience75001XIAP (Bir3), His-tag50 µgbpsbioscience80401XIAP, FLAG-tag20 µgbpsbioscience

Bpsbioscience/NF-κB Luciferase Reporter (Luc) - Jurkat Recombinant Cell Line/60651/2 vials