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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Species: Human
Host Species: E. coli
MW: 44 kDa
Genbank #: NM_020382
Tag(s): N-terminal GST-tag
a.a: 195–352
Background:
SETD8 or SET domain containing lysine methyltransferase 8 is a lysine methyltransferase that monomethylates p53 in human cell lines. The monomethylation by SETD8 suppresses p53-mediated transcriptional activation of highly responsive target genes, such as p21 (CDKN1A) and PUMA (BBC3) but it has little influence on weak p53 targets. Depletion of SET8 augmented the proapoptotic and checkpoint activation functions of p53, and SETD8 expression is downregulated during DNA damage. SETD8 is a Wnt signaling mediator and is recruited by LEF1/TCF4 to regulate the transcription of Wnt-activated genes, possibly through H4K20 monomethylation at the target gene promoters.
Description:
Human SET8 (GenBank Accession No. NM_020382), a.a. 195-352 with N-terminal GST-tag, MW = 44 kDa, expressed in an E. coli expression system.
UniProt
Q9NQR1
Synonym(s):
PR-Set7, SET07, SET8, SETD8
Assay Conditions:
50 µl reaction mix (50 mM Tris, pH 8.8, 1 mM EDTA, 1 mM DTT, 40 µM S-adenosylhomocysteine, and 0-6 µg SET8) is added to the wells coated with the substrate. Incubate for 2 hr. Add antibody against methylated residue of histone H4, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminsecent substrates and read luminescence.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Fang,J. et al., Curr. Biol. 12 (13), 1086-1099 (2002).
2. Yin,Y. et al., Cell Cycle 7 (10), 1423-1432 (2008).
2. Yin,Y. et al., Cell Cycle 7 (10), 1423-1432 (2008).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
