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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Species:Human
HostSpecies:E.coli
MW:17kDa
Genbank#:NM_003336
Tag(s):N-TerminalHis-Tag
Background:
UBE2Aorubiquitin-conjugatingenzymeE2AisamemberoftheE2ubiquitin-conjugatingenzymefamilywhichisrequiredforpost-replicativeDNAdamagerepairandplaysanimportantroleinvariouscellularprocesses.RAD18andRAD5playacentralroleinmediatingphysicalcontactsbetweenthemembersoftheRAD6pathway.UBE2AisspecificallyimportantduringchroniclowdoseultravioletexposuretopreventcounterproductiveDNAcheckpointactivationandallowcellstoproliferatenormally.LossoftheRAD6error-freePRRpathwayresultsinDNAdamagecheckpoint-inducedG2arrestinCLUV-exposedcells.
Description:
Recombinantfull-lengthHumanUBE2A,alsoknownasHHR6A,MRXS30,MRXSN,RAD6A,andUBC.GenbankAccessionNo.NM_003336,withanN-terminalHis-tag,expressedinE.Colicellsviaabaculovirusexpressionsystem.MW=17kDa.
Synonym(s):
HHR6A,MRXS30,MRXSN,RAD6A,UBC
Formulation:
50mMsodiumphosphate,pH7.0,300mMNaCl,150mMimidazole,0.1mMPMSF,0.25mMDTT,and25%glycerol.
Storage/StABIlity:
Atleast6monthsat–70°C.Avoidfreeze/thawcycles.
Application(s):
Usefulforthestudyofubiquitinenzymekinetics,andasapositivecontrolforWesternblotstudies.
Reference(s):
1.Mondal,S.,etal.,Anal.Biochem.2016;510:41-51.
2.Hishida,T.,etal.,Nature.2009;457:612-615.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Ubiquitination
ProductType:
ActivatingEnzyme
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