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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Species:S.aureus
HostSpecies:E.coli
MW:22kDa
Genbank#:AF162687
Tag(s):N-terminalHis-tag
a.a:26-206(end)
Background:
StaphylococcalSortaseAisabacterialtranspeptidasethatcovalentlyattachesproteinstothebacterialcellwallbycleavingbetweenthreonineandglycineatanLPXTGrecognitionmotifandcatalyzestheformationofanamidebondbetweenthecarboxyl-groupofthreonineandtheamino-groupofthecell-wallpeptidoglycan.Thischemistryhasbeenexploitedasamolecular"stapler"tosite-specificallylinkproteinswiththeC-terminalLPETGXmotiftootherproteinsormoleculespossessingaglycineoraminomethylenemotif.Thisreactionisapplicabletoproteinsinsolutionandonthelivingcellsurface.
Description:
StaphylococcusaureussortaseAHexamutant(P94R,D124G,D160N,D165A,K190E,K196T),a.a26-end,GenBankAccessionNo.AF162687withN-terminalHistag,MW=22kDa,expressedinanE.coliexpressionsystem.
Synonym(s):
SrtA,sortaseA
Formulation:
40mMTrispH8.0,110mMNaCl,2.2mMKCl,400mMimidazoleand20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.
Application(s):
Suitableforusewithinvitroproteinligationreactions.
Reference(s):
1.Chen,I.etal.,ProcNatl.Acad.Sci.(USA).2011;108(28):11399-11404.
2.Kappel,K.,etal.,ProteinSci.2012Dec;21(12):1858-71.
2.Kappel,K.,etal.,ProteinSci.2012Dec;21(12):1858-71.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
Other
ProductType:
Recombinantprotein
Datashownislot-specific.Contactusforspecificinformationonotherlots.


