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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Background:
TheQuickPCR™CloningKitisasimpleandhighlyefficientmethodtoinsertanygeneorDNAfragmentintoavector,withouttime-consumingligationsteps.MultiplePCRinsertscanbeaddedinasinglestep.Theprocedureiseasy:aprimersethomologoustothegeneofinterestandthevectorareusedtoamplifyaPCRproduct.ThispurifiedPCRproductisincubatedwithrestrictionenzyme-digestedvectorandReagentAoftheQuickPCR™CloningKit.Followingabrief(5minute)incubationwithReagentB,theannealedvectorisreadyfortransformation.
Description:
TheBPSBioscienceQuickPCR™cloningkitallowsfortheinsertionofmultipleDNAfragmentsintothedesiredvectorinasinglereaction.Noligationstepisrequired,savingtimeandeffort.Themethodisfast,simple,andefficient.GofromPCRproducttotransformingcellsinunderanhour!SimplymixthepurifiedPCRproductwiththevectorina1:1molarratio(TotalDNA:Vector)andfollowtheprotocol.Efficienciesof>90%areroutinelyobtained.Availablewithorwithoutcompetentcellsforevengreaterefficiencyandflexibility.
SuppliedAs:
Components:
ReagentA
ReagentB
BufferA
10XBSA(0.1%w/v)
ReagentA
ReagentB
BufferA
10XBSA(0.1%w/v)
Efficiency:
Efficienciesof>90%aretypical.
Format:
assaykit
Instructionsforuse:
Seedatasheetfordetailedprotocol.
Storage/StABIlity:
Kitcomponentsmaybestoredat-20°Corbelow.Avoidfreeze/thawcycles.Uponfirstthaw,storeallreagentsinsingleusealiquots.Stableforatleast6monthsfromdateofreceipt,whenstoredasdirected.
Application(s):
KitisdesignedforcloninganygeneorDNAfragmentintoanyvectorinaquick,single-stepreaction.Canbeusedwithmultipleinsertsinasinglereaction.Alsosuitableforfixingoraddingpointmutations,insertions,ordeletions.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Mol.Biol.Reag./Cloning
ProductType:
PCRKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.


