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ChIPresultsobtainedwiththemonoclonalantibodydirectedagainstHDAC3
ChIPassayswereperformedusinghumanHeLacells,themonoclonalantibodyagainstHDAC3(Cat.#25289)andoptimizedPCRprimersetsforqPCR.ChIPwasperformedonshearedchromatinfrom10,000cells.Atitrationoftheantibodyconsistingof1,2,5,and10µgperChIPexperimentwasanalyzed.IgG(5µg/IP)wasusedasnegativeIPcontrol.QPCRwasperformedwithprimersforthepromotersoftheactivegenesc-fosandGAPDH,andforthecodingregionofp21,aknowntargetgeneofHDAC3.Figure4showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis).
ImmunofluorescenceusingthemonoclonalantibodydirectedagainstHDAC3
HeLacellswerestainedwiththeantibodyagainstHDAC3(Cat.#25289)andwithDAPI.Cellswerefixedwith4%formaldehydefor10min.andblockedwithPBS/TX-100containing5%normalgoatserumand1%BSA.ThecellswereimmunofluorescentlylabelledwiththeHDAC3antibody(left)diluted1:500inblockingsolutionfollowedbyananti-mouseantibodyconjugatedtoAlexa594.ThemiddlepanelshowsstainingofthenucleiwithDAPI.Amergeofthetwostainingsisshownontheright.
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
IF(1:500)


