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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Background:
Hsp90βisamolecularchaperonewithessentialfunctionsinmaintainingtransformation.InhibitionofHsp90βfunctionshasbeenshowntoplayaroleintumOrigenesisanddiseaseprogression.
Description:
TheHSP90β(N-terminal)AssayKitisdesignedforidentificationofHSP90βinhibitorsusingfluorescencepolarization.TheassayisbasedonthecompetitionoffluorescentlylabeledgeldanamycinforbindingtopurifiedrecombinantHSP90β.ThekeytotheHSP90βAssayKitisthefluorescentlylabeledgeldanamycin.Usingthiskit,onlyonesimplesteponamicrotiterplateisrequiredforHSP90βreactions.ThefluorescentlylabeledgeldanamycinisincubatedwithasamplecontainingHSP90βenzymetoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.
UniProt
P08238
Synonym(s):
HSP90b,HeatShockProtein90b,HSP90beta,HSP90β
SuppliedAs:
TheHSP90βinhibitorscreeningassaykitcomesinaconvenient384-wellformat,withpurifiedHSP90βenzyme,fluorescentlylabeledgeldanamycinandHSP90assaybufferfor384enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
COMPONENTS:
Instructionsforuse:
Seedatasheetfordetailedprotocol.
Storage/StABIlity:
Stableforatleast12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.Kim,J.,etal.JBiomolScreening2004,9(5):375-381.
2.Howes,R.,etal.AnalBiochem2006,350:202-213.
2.Howes,R.,etal.AnalBiochem2006,350:202-213.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
HeatShockProteins
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.


