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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description:
TheHSP90β(C-Terminal)TR-FRETassaykitisdesignedtomeasuretheinhibitionofHSP90αbindingtoitsproteintargetPPID(alsoknownasCyclophilinDorCyp40)inahomogeneous,384reactionformat.Thisassayrequiresnotime-consumingwashingsteps,makingitespeciallysuitableforhighthroughputscreeningapplications.Theassayprocedureisstraightforwardandsimple.Asamplecontainingterbium-labeleddonor,dye-labeledacceptor,HSP90β,substrate,andaninhibitorisincubatedfor2hours.Theprotein-proteininteractionisthenassayedbymeasuringtheTR-FRETbetweenthePPIDandHSP90βusingafluorescencereader.
UniProt
P08238
Synonym(s):
HSP90b,HeatShockProtein90b,HSP90beta,HSP90β
SuppliedAs:
Comesinahomogeneous,384reactionformat
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Allan,R.K.etal.J.Biol.Chem.2006281(11):7161-71
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
FluorescentmicroplatereadercapableofmeasuringTimeResolvedFluorescenceResonance
EnergyTransfer(TR-FRET)
Adjustablemicropipettorandsteriletips
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
HeatShockProteins/AssayKits
ProductType:
AssayKits
Datashownislot-specific.Contactusforspecificinformationonotherlots.


