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Dotblotanalysisusingtheantibodydirectedagainst3-mC
Todemonstratethespecificityoftheantibodyagainst3-mC(cat.No.25200),aDotBlotanalysiswasperformedusingsyntheticoligonucleotidescontainingdifferentmodifiedbases.150and50pmoloftherespectiveoligo’swerespottedonthemembrane.Theantibodywasdiluted1:400inPBS-Tcontaining10%skimmedmilkand1%BSA.Figure1showsahighspecificityoftheantibodyfortheoligonucleotidewiththe3-methylcytosinemodification.
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.


