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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Species:Human
HostSpecies:E.coli
MW:27kDa
Genbank#:P10415
Tag(s):C-terminalHis-tag
a.a:1–211
Background:
Suppressesapoptosisinavarietyofcellsystemsincludingfactor-dependentlymphohematopoieticandneuralcells.RegulatescelldeathbycontrollingthemitochondrialmembranepermeABIlity.Appearstofunctioninafeedbackloopsystemwithcaspases.Inhibitscaspaseactivityeitherbypreventingthereleaseofcytochromecfromthemitochondriaand/orbybindingtotheapoptosis-activatingfactor(APAF-1).
Description:
HumanBcl-2(GenBankAccessionNo.P10415),a.a.1-211withC-terminalHis-tag,MW=27kDa,expressedinanE.coliexpressionsystem.
UniProt
P10415
Synonym(s):
Bcl,Bcl2,Bcelllymphoma2
AssayConditions:
FollowingtheprotocolforBPSCat.#50222.Briefly,BCL-2wascombinedwithBCL-2peptideligandinTR-FRETassaybufferalongwithanti-HisTbdonoranddyelabeledacceptor.VaryingconcentrationsofABT199wereaddedandthereactionwasincubatedatroomtemperaturefor2hoursfollowedbyfluorescentintensitymeasurements.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and200mMimidazole
Format:
Aqueousbuffersolution
Application(s):
Usefulforthestudyofapoptosis,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.LabiV.etal.,CellDeathDiffer.2008Jun;15(6):977-87.
2.RongY.P.etal.,MolCell.2008Jul25;31(2):255-65.
2.RongY.P.etal.,MolCell.2008Jul25;31(2):255-65.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Apoptosis
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.


