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蚂蚁淘在线 / 品牌中心 / Usbio / Usbio/172120-AF-HRP小鼠抗Lambda轻链（Bence-Jones蛋白，BJP，IGLC 1/2/3，Mcg标记物，副蛋白）（HRP）/172120-AF-HRP/100ul
商品描述
ThisMAbisspecifictolamBDalightchainofimmunoglobulinandshowsnocross-reactionwithlambdalightchainoranyofthefiveheavychains.Inmammals,thetwolightchainsinanantibodyarealwaysidentical,withonlyonetypeoflightchain,kappaorlambda.TheratioofKappatoLambdais70:30.However,withtheoccurrenceofmultiplemyelomaorotherB-cellmalignanciesthisratioisdisturbed.Antibodytothelambdalightchainisreportedlyusefulintheidentificationofleukemias,plasmacytomas,andcertainnon-Hodgkin'slymphomas.Demonstrationofclonalityinlymphoidinfiltratesindicatesthattheinfiltrateismalignant.
Applications:|SuitableforuseinELISA,WesternBlot,Immunoprecipitation,Immunohistochemistry.Otherapplicationsnottested.
RecommendedDilution
WesternBlot:0.5-1ug/mlImmunoprecipitation:1-2ug/500ugproteinlysateImmunohistochemistry(Frozen&Formalin-fixed):0.5-1ug/mlfor30minutesatRT(Stainingofformalin-fixedtissuesrequiresboilingtissuesectionsin10mMCitrateBuffer,pH6.0,for10-20minfollowedbycoolingatRTfor20minutes)Optimaldilutionforaspecificapplicationshouldbedetermined.
PositiveControl
293T,RajiorhPBLcells.TonsilorSpleen
StorageandStABIlity
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.LabeledwithhorserADIshperoxidase(HRP).SodiumazideisapotentinhibitorofperoxidaseandshouldnotbeaddedtoHRPconjugates.
Note:Applicationsarebasedonunconjugatedantibody.
